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Thermo Scientific™ Fluorescein-5-Maleimide

Activated derivatives of green-fluorescent fluorescein dye to label antibodies, proteins and other molecules for fluorescence imaging (ex 494; em 518).

Brand:  Thermo Scientific™ 62245


Product Code. 10078847

  • 2015.00 NOK / 25mg

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Description

Description

Thermo Scientific™ FITC and Fluorescein Labeling Reagents and Kits are high-performance derivatives of fluorescein dye, activated for easy and reliable labeling of antibodies, proteins and other molecules for use as fluorescent probes.

Fluorescein dyes are used in wide-ranging applications including fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA. FITC and NHS-fluorescein are reactive toward primary amine groups on proteins, peptides and other biomolecules, while fluorescein-5-maleimide and 5-IAF react with free sulfhydryls on cysteine residues.Most activated fluorescein derivatives are mixture of isomers with reactive groups attached at the 5- and 6-positions of the bottom ring. The properties of these isomers are indistinguishable in terms of excitation and emission spectra and for protein applications there is no need to isolate a specific isomer. The single isomer is used in high resolution separation techniques such as sequencing and capillary electrophoresis.

Highlights:

  • Amine-specific labeling: the NHS-ester or isothiocyanate efficiently labels antibodies and other purified proteins at primary amines (lysine side chains) Optimized procedure: following the standard protocol results in antibodies with excellent dye:protein ratios for optimum activity and fluorescence Single-use fluors: no need to weigh tiny amounts powder; kits contain single-use vials of reagent Efficient purification: kits include purification resin and easy-to-use spin columns, ensuring rapid and efficient removal of non-reacted dye and excellent protein recovery
TRUSTED_SUSTAINABILITY
Specifications

Specifications

25 mg
DMF (Dimethylformamide)
Thiol
FITC (Fluorescein)
Pierce™
494
Maleimide
Store desiccated at -20°C, protect from moisture
Maleimide
Classic Dyes
518
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Product Information

Manuals

User Guide: Fluorescein-5-Maleimide Version: Jan. 2015 File size: 61.9 kB
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A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.

5 results found
Lot Number Certificate Type Date Catalog Number
Lot NumberZL4570337 Certificate TypeCertificate of Analysis Date12/02/2025 Catalog Number62245
Lot NumberZI4451605 Certificate TypeCertificate of Analysis Date15/10/2024 Catalog Number62245
Lot NumberZF4332981 Certificate TypeCertificate of Analysis Date28/06/2024 Catalog Number62245
Lot NumberZE4276733 Certificate TypeCertificate of Analysis Date17/05/2024 Catalog Number62245
Lot NumberZC4219255 Certificate TypeCertificate of Analysis Date15/03/2024 Catalog Number62245
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