missing translation for 'onlineSavingsMsg'
Learn More

Thermo Scientific™ Pierce™ EDC, No-Weigh™ Format

Immobilize or conjugate proteins using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (CAS 25952-53-8), a water-soluble, carboxyl-to-amine crosslinker.

Brand: Thermo Scientific™ 77149

577.60 NOK valid until 2025-04-30
Use promo code "24155" to get your promotional price.

View more versions of this product

Product Code. 11844071

624.00 NOK / 10mg

Please sign in to purchase this item. Need a web account? Register with us today!

This item is not returnable. View return policy

Description

Thermo Scientific™ Pierce EDC is a water-soluble carbodiimide crosslinker that activates carboxyl groups for spontaneous reaction with primary amines, enabling peptide immobilization and hapten-carrier protein conjugation.

EDC is the abbreviation for 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride, a crosslinker that has been used in diverse applications such as forming amide bonds in peptide synthesis, attaching haptens to carrier proteins to form immunogens, labeling nucleic acids through 5Ãƒâ€ phosphate groups and creating amine-reactive NHS-esters of biomolecules. EDC reacts with a carboxyl to form an amine-reactive O-acylisourea intermediate. If this intermediate does not encounter an amine, it will hydrolyze and regenerate the carboxyl group. In the presence of N-hydroxysulfosuccinimide (Sulfo-NHS, Part No. 24510), EDC can be used to convert carboxyl groups to amine-reactive Sulfo-NHS esters.

Highlights:

Zero-length crosslinker
Numerous chemical conjugates can be synthesized via available –COOH and –NH2 groups
Amide bond formed provides a neutral linkage, which is ideal for preparing peptides and antigens
Adding Sulfo-NHS (Part No. 24510) enhances the coupling reaction at physiological pH conditions
Reactive groups: carbodiimide
Reactive toward: carboxyl and amino groups

Specifications

Chemical Reactivity	Amine-Carboxyl
Description	EDC
PEGylated	No
Content And Storage	Upon receipt store desiccated at -20°C.
Shipping Condition	Wet Ice
Labeling Method	Chemical Labeling
Reactive Moiety	Carbodiimide
Form	Powder
Solubility	Water
Water Soluble	Yes

Cleavable	No
Molecular Weight (g/mol)	191.7
Spacer Arm Length	0.0 Å
Cell Permeability	No
Product Line	Pierce™
Crosslinker Type	Heterobifunctional
Spacer	Short (<10 Å)
Quantity	10 mg
Format	Standard, Single-use, Premium-grade
Product Type	Crosslinker

Product Suggestions Safety and Handling

Safety and Handling

Product Identifier

EDC 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochlor

Signal Word

Warning

Hazard Category

Serious eye damage/eye irritation Category 2
Skin corrosion/irritation Category 2
Specific target organ toxicity Category 3

Hazard Statement

H315-Causes skin irritation.
H319-Causes serious eye irritation.
H335-May cause respiratory irritation.

Precautionary Statement

P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
P264-Wash hands thoroughly after handling.
P271-Use only outdoors or in a well-ventilated area.
P280-Wear protective gloves/protective clothing/eye protection/face protection.
P302+P352-IF ON SKIN: Wash with plenty of water/soap.
P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P312-Call a POISON CENTER/doctor/if you feel unwell.
P332+P313-If skin irritation occurs: Get medical advice/attention.
P337+P313-If eye irritation persists: Get medical advice/attention.
P362+P364-Take off contaminated clothing and wash it before reuse.
P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

MIXTURE LIST-Contains : 1,3-Propanediamine,N-(ethylcarbonimidoyl)-N,N-di methyl-, monohydrochloride

Documents

Scientific Resources

Application Notes

Tech Tip: Modify and label oligonucleotide 5â€™-phosphate groups File size: 55.1 kB

Tech Tip: Block amine groups to prevent polymer formation in immunogen production File size: 155.7 kB

Product Information

Manuals

User Guide: EDC Version: MAN0017125 Rev. A.0 (23May2017) File size: 61.1 kB

Certificates

A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.

Lot Number

5 results found

Lot Number	Certificate Type	Date	Catalog Number
Lot NumberZL390598	Certificate TypeCertificate of Analysis	Date31/01/2025	Catalog Number77149
Lot NumberZJ387516	Certificate TypeCertificate of Analysis	Date01/11/2024	Catalog Number77149
Lot NumberZG387515	Certificate TypeCertificate of Analysis	Date07/08/2024	Catalog Number77149
Lot NumberZG386923	Certificate TypeCertificate of Analysis	Date19/07/2024	Catalog Number77149
Lot NumberZE386605	Certificate TypeCertificate of Analysis	Date11/06/2024	Catalog Number77149

Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

Thermo Scientific™ Pierce™ EDC, No-Weigh™ Format > 10 mg

Your Name

Email Address *

Type of Issue *

Detailed Description of Issue/Request *

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.

For Research Use Only. Not for use in diagnostic procedures.

Spot an opportunity for improvement?Share a Content Correction