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Thermo Scientific™ Zeba™ Dye and Biotin Removal Spin Columns and Filter Plates, 0.5–10 mL

Product Code. 16627182 Shop All Thermo Scientific Products
25 Columns, Spin Column, 2 mL, Each
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Quantity:
2 Plates
2 x 2 Plates
2 x 25 Columns
25 Columns
5 Columns
5 x 5 Columns
Format:
96-well Filter Plate
Spin Column
Volume (Metric):
0.5 mL
10 mL
2 mL
5 mL
96-well
Unit Size:
Each
Pack of 2
Pack of 25
Pack of 4
Pack of 5
Pack of 50
This item is not returnable. View return policy
This item is not returnable. View return policy

Enable fast and efficient removal of non-reacted fluorescent dyes, biotin, reducing agents, and crosslinkers from protein samples with Thermo Scientific Pierce Zeba Dye and Biotin Removal columns and filter plates.

Quickly remove non-reacted fluorescent dyes, biotin, reducing agents, and crosslinkers from protein samples with a 7K MWCO in fewer than 15 minutes with Thermo Scientific Pierce Zeba Dye and Biotin Removal columns and filter plates.

Removing free-dye after a labeling reaction is often difficult and time-consuming but is essential for accurate determination of dye-to-protein ratios. Pierce Zeba Dye and Biotin Removal resin is highly specialized to produce exceptional protein recoveries while effectively removing non-conjugated fluorescent dyes, biotinylation reagents, reducing agents, and crosslinkers. When using the appropriate amount of sample and working within buffer conditions, these columns and filter plates can successfully remove almost any fluorescent dye. Even dilute protein samples (25 ÎĽg/mL) can be successfully processed to remove dyes, biotin, reducing agents, and crosslinkers and to efficiently recover proteins (>7 kDa). These products are not recommended for desalting or buffer exchange.

Features of Zeba Dye and Biotin Removal columns and filter plates include:
• Versatile—removes unreacted fluorescent dyes, biotinylation reagents, crosslinkers, and reducing agents from protein solutions
• High recovery—low-binding resin maximizes protein recovery
• Easy-to-use—no cumbersome column preparation or equilibration
• Fast—small-molecule removal and protein recovery in less than 15 minutes
• Flexible—available in spin columns and filter spin plates for a range of needs

Sample capacities
• 0.5-mL spin column: 50 to 120 μL sample
• 2-mL spin column: 400 to 700 μL sample
• 5-mL spin column: 1 to 2 mL sample
• 96-well filter plate: 40 to 100 μL sample (per well)

Available formats
• Spin columns: 0.5, 2, 5, and 10 mL
• 96-well filter plates: pre-dispensed; compatible with centrifugation for manual or automated purification; enable fast, consistent well-to-well and plate-to-plate reproducibility for small-scale, high-throughput clean-ups

Applications
• Ideal for post-reaction clean-up of samples
• To effectively remove non-conjugated dye, biotin, reducing agents, and crosslinkers with exceptional protein recovery

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Store in refrigerator (2–8°C).
Shipping Condition Room Temperature
Format Spin Column
Column Type Proprietary Resin
Product Line Zeba™
Quantity 25 Columns
Volume (Metric) 2 mL
Sample Volume (Metric) 400 to 700 ÎĽL
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