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Thermo Scientific™ Pierce™ Microplate BCA Protein Assay Kit - Reducing Agent Compatible

Reducing agent-compatible version of our popular BCA Reagent Kit to measure protein concentration in samples containing DTT or BME thiol-reductants.

Brand: Thermo Scientific™ 23252

1790.08 NOK valid until 2025-03-21
Use promo code "24111" to get your promotional price.

Product Code. 10209523

2685.00 NOK / 1 set

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To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”24111” to get your promotional price

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Description

This reducing agent-compatible (RAC) version of the Thermo Scientific™ Pierce BCA Protein Assay provides accurate determination of protein concentration in samples that contain dithiothreitol (DTT), 2-mercaptoethanol (BME), TCEP and other disulfide reducing agents.

The BCA Protein Assay Kit - Reducing Agent Compatible (BCA-RAC) provides all of the advantages of the original BCA Assay, plus compatibility with disulfide reducing agents at concentrations routinely used in protein sample buffers. This special adaptation of the popular Pierce BCA Protein Assay method enables accurate protein concentration measurement for samples containing DTT, 2-ME or TCEP. Available in tube and microplate formats, these reducing agent compatible (RAC) BCA Kits extend the already broad reagent compatibility of the BCA Protein Assay protocol to include nearly all types of components commonly present in protein research samples.

Highlights:

Compatible – assay samples that contain up to 5mM DTT, 35mM BME, or 10mM TCEP
BCA technology – only a slight modification of the standard BCA Protein Assay protocol (15-minute incubation with Compatibility Reagent); no precipitation steps required
Small samples – requires only 25μL (standard kit) or less than 10μL (microplate kit) of sample
Colorimetric – measure with a standard spectrophotometer or plate reader (562nm)
Excellent uniformity – exhibits less protein-to-protein variation than dye-binding methods
High linearity – linear working range for BSA equals 125 to 2000μg/mL

Includes:

BCA Reagents A and B, Compatibility Reagent, Reconstitution Buffer, and Albumin Standard (2mg/mL); Microplate kit also includes assay-compatible 96-well microplates

Specifications

Content And Storage	Sufficient For: 980 microplate assays with 9 μL samples • BCA Reagent A, 250 mL • BCA Reagent B, 25 mL • Compatibility Reagent, 48 x 10 mg • Reconstitution Buffer, 15 mL • Albumin Standard Ampules, 2mg/mL, 10 x 1 mL • 96-Well Microplates, 20 plates Store at room temperature
Assay	BCA Assay
Product Line	Pierce™
Specificity	Not Target-Specific
For Use With (Application)	Solution-based Detection, Absorbance
Quantity	275 mL

Description	Pierce BCA Microplate Protein Assay Kit, Reducing Agent Compatible (RAC)
Detection Method	Colorimetric
Product Type	Protein Quantitation Assay
Sufficient For	980 Microplate Assays
For Use With (Equipment)	Spectrophotometer, Microplate Reader

Product Suggestions Safety and Handling

Safety and Handling

Product Identifier

Pierce Microplate BCA Protein Assay Kit - Reducing Agent Compatible

Signal Word

Danger

Hazard Category

Acute toxicity Category 3
Respiratory sensitiser Category 1
Skin sensitiser Category 1
ACUTE AQUATIC Acute 1
LONG-TERM AQUATIC HAZARD Chronic 2

Hazard Statement

H301-Toxic if swallowed.
H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
H317-May cause an allergic skin reaction.
H400-Very toxic to aquatic life.
H411-Toxic to aquatic life with long lasting effects.

Precautionary Statement

P280-Wear protective gloves/protective clothing/eye protection/face protection.
P284-¢In case of inadequate ventilation! wear respiratory protection.
P273-Avoid release to the environment.
P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
P391-Collect spillage.
P302+P352-IF ON SKIN: Wash with plenty of water/.
P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
P405-Store locked up.
P501-Dispose of contents/container to .

Supplemental information

MIXTURE LIST-Contains : sodium carbonate, ¢2, 2'-Biquinoline!-4,4'-dicarboxylic acid, sodium salt (1:2), Sulfuric acid copp

Documents

Scientific Resources

Application Notes

Tech Tip: Protein quantitation assay compatibility table File size: 104 kB

Tech Tip: How to use a protein assay standard curve File size: 65.6 kB

Product Information

Manuals

User Guide: Pierce Microplate BCA Protein Assay Kit – Reducing Agent Compatible Version: Jan. 2015 File size: 72.3 kB

Certificates

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Lot Number

5 results found

Lot Number	Certificate Type	Date	Catalog Number
Lot NumberZL402603	Certificate TypeCertificate of Analysis	Date31/01/2025	Catalog Number23252
Lot NumberZL402603A	Certificate TypeCertificate of Analysis	Date31/01/2025	Catalog Number23252
Lot NumberZJ402149	Certificate TypeCertificate of Analysis	Date16/12/2024	Catalog Number23252
Lot NumberZJ402149A	Certificate TypeCertificate of Analysis	Date16/12/2024	Catalog Number23252
Lot NumberZI399434A	Certificate TypeCertificate of Analysis	Date15/10/2024	Catalog Number23252

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Thermo Scientific™ Pierce™ Microplate BCA Protein Assay Kit - Reducing Agent Compatible > Microplate assay kit; 275mL reagent for 980 microplate wells

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