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Thermo Scientific™ Phusion™ HF and GC Buffer Packs

5X Phusion Green buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel.

269.00 NOK - 311.00 NOK

Specifications

Polymerase Phusion High-Fidelity DNA Polymerase
Shipping Condition Wet or Dry Ice
For Use With (Application) Standard PCR, High-fidelity PCR
Starting Material DNA
Volume 4 x 1.5 mL
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Products 6
Product Code Brand Product Type Color Price Quantity & Availability  
Product Code Brand Product Type Color Price Quantity & Availability  
10492088
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Thermo Scientific™
F518L
HF Buffer Pack Colorless
271.00 NOK
Pack of 4
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10584578
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Thermo Scientific™
F519L
GC Buffer Pack Colorless
270.00 NOK
Pack of 4
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10226009
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Thermo Scientific™
F520L
Detergent-free FF Buffer Pack Colorless
308.00 NOK
Pack of 4
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10333609
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Thermo Scientific™
F521L
Detergent-free GC Buffer Pack Colorless
311.00 NOK
Pack of 4
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13525170
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Thermo Scientific™
F538L
HF Buffer Pack Green
270.00 NOK
Pack of 4
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13535170
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Thermo Scientific™
F539L
GC Buffer Pack Green
269.00 NOK
Pack of 4
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Description

Description

The error rate of Phusion DNA Polymerase in HF Buffer (4.4 x 10-7) is lower than that in GC Buffer (9.5 x 10-7). Therefore, the HF Buffer should be used as the default buffer for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures. For applications such as microarray or DHPLC, where the DNA templates need to be free of detergents, we recommend using detergent-free reaction buffers.

5X Phusion Green Buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffers do not interfere with the enzyme performance and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Two optimized buffers are available for Thermo Scientific Phusion High-Fidelity DNA Polymerases:

  • 5X Phusion HF Buffer (F-518L, F-538L (Phusion Green), F-520L (Detergent-free))
  • 5X Phusion GC Buffer (F-519L, F-539L (Phusion Green), F-521L (Detergent-free))
Specifications

Specifications

Phusion High-Fidelity DNA Polymerase
Standard PCR, High-fidelity PCR
4 x 1.5 mL
Wet or Dry Ice
DNA
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SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.