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Thermo Scientific™ Phusion Blood Direct PCR Master Mix

Thermo Scientific Phusion Blood Direct PCR Master Mix is designed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR.

Brand: Thermo Scientific™ F175L

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Product Code. 15290114

4250.00 NOK / 500 reactions

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Description

Thermo Scientific Phusion Blood Direct PCR Master Mix is designed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR. The modified Phusion Hot Start II High-Fidelity DNA Polymerase is resistant to main PCR inhibitors present in blood and retains its activity at blood concentrations of up to 40% in the PCR reaction.

This accurate proofreading DNA polymerase makes the Phusion Blood Direct PCR Master Mix an ideal choice for demanding applications, such as cloning. Phusion Blood Direct PCR Master Mix also allows analysis of blood samples with different preservatives (e.g., heparin, EDTA, citrate), storage conditions, and from various species.

Highlights

Direct PCR allows sample to be added directly to PCR reaction, reducing time-consuming and expensive DNA purification steps
Available Dilution & Storage protocol allows several PCR reactions from one sample
Specially engineered Phire Hot Start II DNA polymerase for extremely short PCR protocol times
Master mix format with premixed gel loading dye minimizes risk of cross-contamination, reduces sample handling, and allows direct loading on gel

Applications

Genotyping
Transgene detection
Knockout analysis
Sequencing
Cloning

Notes

Annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases.

Specifications

Content And Storage	• 2X Phusion Blood Direct PCR Master Mix, 5 x 1 mL • Universal Control Primer Mix, 40 μL • 50 mM EDTA, 0.5 mL • 50 mM DMSO, 0.5 mL • 50 mM MgCl₂, 1.5 mL • Nuclease-free water, 4 x 1.25 mL • O'GeneRuler Express DNA Ladder, 50 μg (sufficient for 100 applications) Store at -20°C.
PCR Method	Direct PCR
Reaction Speed	Fast
Product Type	Direct PCR Master Mix
Shipping Condition	Wet or Dry Ice
Concentration	2X
No. of Reactions	500 Reactions
Reaction Format	SuperMix or Master Mix
Starting Material	DNA

GC-Rich PCR Performance	High
Polymerase	Phusion Blood II DNA Polymerase
Includes Label or Dye	Yes
Quantity	500 reactions
For Use With (Application)	Genotyping
Hot Start	Built-In Hot Start
Overhang	Blunt
Sample Type	Blood

Product Suggestions Documents

Documents

Scientific Resources

Application Notes

Brochure: Thermo Scientific Direct PCR—amplify without purification File size: 1.9 MB

Brochures

Phusion Blood Direct PCR Master Mix and Kit—recommended blood amounts File size: 33.2 kB

Reference Materials

LabAid: Direct PCR solutions File size: 65.2 kB

Product Information

Manuals

User Guide: Phusion Blood Direct PCR Master Mix Version: MAN0012900 Rev. B.0 (05Dec2016) File size: 220.6 kB

Certificates

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Lot Number

5 results found

Lot Number	Certificate Type	Date	Catalog Number
Lot Number2812572	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog NumberF175L
Lot Number2835237	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog NumberF175L
Lot Number2903867	Certificate TypeCertificate of Analysis	Date28/01/2025	Catalog NumberF175L
Lot Number2923009	Certificate TypeCertificate of Analysis	Date27/01/2025	Catalog NumberF175L
Lot Number3084139	Certificate TypeCertificate of Analysis	Date27/01/2025	Catalog NumberF175L

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