missing translation for 'onlineSavingsMsg'
Learn More

Molecular Probes™ pHrodo™ Red Avidin (Fluorogenic pH Sensor)

pHrodo™ Red Avidin (Fluorogenic pH Sensor)

Brand: Molecular Probes™ P35362

3898.80 NOK valid until 2025-03-28
Use promo code "24107" to get your promotional price.

Product Code. 11612159

5270.00 NOK / 1mg

Quantity

In Stock
to see stock.

Explore more special offers

Add to basket

This item is not returnable. View return policy

Description

pHrodo™ Red Avidin is a conjugate of avidin and pHrodo™ Red dye, a novel, fluorogenic dye that dramatically increases in fluorescence as the pH of its surroundings become more acidic. pHrodo™ Red dye conjugates are non-fluorescent outside the cell, but fluoresce bright red in phagosomes, making them ideal tools for studies ranging from phagocytosis of bioparticles to receptor internalization. Original pHrodo™ and pHrodo™ Red dye are the same molecule.

With pHrodo™ Red Avidin Fluorogenic pH Sensor You Get:
Specific detection of phagocytosis and endocytosis
Faster staining
More accurate results in sensitive experiments
Multiplexing capability with green dyes

Validation in flow cytometry, imaging, HTS, and HCS

Get Better Results in Endocytosis Studies
Endocytosis and phagocytosis underly physiological events ranging from host-pathogen response to receptor desensitization via internalization as is common in the GPCR class of receptors). Once internalized, material can be acidified in endosomes or lysosomes. Our pHrodo™ Red dye fluorogenic pH sensitive probe, when conjugated to target molecules, allows direct visual monitoring of material entering these acidic compartments. At neutral and basic pH values, pHrodo™ Red dye emission is at its minimum; with acidification, pHrodo™ Red dye emission is increased by as much as 8-fold (at pH 4). The red fluorescence of pHrodo™ Red Avidin is ideal for multiplexing applications with green-fluorescent compounds such GFP, Fluo-4, or calcein.

Faster and More Accurate Staining in Multiple Validated Applications
pHrodo™ Red Avidin offers reduced signal variability and improved timing compared to ratiometric dyes like BCECF and SNARF, and the lack of fluorescence outside the cell eliminates the need for wash steps and quencher dyes. The fluorescence of pHrodo™ Red dye is dimmed by complete media and whole blood, further improving the signal-to-noise ratio. pHrodo™ Red has been cited in over 150 publications and is validated in flow cytometry, high throughput screening (HTS), high content screening (HCS), and other imaging applications.

Easy Biotinylation Lets You Enjoy Flexibility in Experimental Design
The pHrodo™ Red Avidin reagent can quickly be coupled to any biotinlyated reagent, including many of our own biotin conjugates of antibodies, proteins, and toxins including transferrin, EGF, and cholera toxin. Alternatively, you can create your own biotinylated target either by using Click-iT™ chemistry to metabolically incorporate an alkyne biotin, or by using any of our biotin labeling kits.

Alternative Conjugation Chemistry to pHrodo™ SE
In cases where the amine reactive pHrodo-SE conjugates show reduced or complete loss of function, biotin and avidin conjugation can be considered using pHrodo™ Red Avidin. In one example, pHrodo-EGF was created using the avidin-biotin approach and shown to bind its receptor and be internalized (Ref 1).

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Reference:

1. Suprynowicz FA, Krawczyk E, Hebert JD, Sudarshan SR, Simic V, Kamonjoh CM, Schlegel R. The human papillomavirus type 16 E5 oncoprotein inhibits epidermal growth factor trafficking independently of endosome acidification. J Virol. 2010 Oct;84(20):10619-29. PubMed PMID: 20686024

Specifications

Conjugate	pHrodo Red
Content And Storage	Store at ≤-20°C Desiccate and protect from light
Product Line	Molecular Probes™, pHrodo™

Quantity	1 mg
Shipping Condition	Room Temperature
Product Type	Avidin

Product Suggestions Documents

Documents

Product Information

Manuals

pHrodo Red Avidin Version: 8 September 2011 File size: 181.3 kB

Certificates

A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.

Lot Number

5 results found

Lot Number	Certificate Type	Date	Catalog Number
Lot Number2892435	Certificate TypeCertificate of Analysis	Date14/06/2024	Catalog NumberP35362
Lot Number2626508	Certificate TypeCertificate of Analysis	Date28/04/2023	Catalog NumberP35362
Lot Number2540595	Certificate TypeCertificate of Analysis	Date31/08/2022	Catalog NumberP35362
Lot Number2407374	Certificate TypeCertificate of Analysis	Date11/11/2021	Catalog NumberP35362
Lot Number2328936	Certificate TypeCertificate of Analysis	Date27/05/2021	Catalog NumberP35362

Special Offers

Multiple Manufacturers

Grow Your Discovery

Fall in love with big savings
Promo Code: 24107
Expires: 2025-03-28

New Offer
Shop Now

Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

Molecular Probes™ pHrodo™ Red Avidin (Fluorogenic pH Sensor) > 1mg

Your Name

Email Address *

Type of Issue *

Detailed Description of Issue/Request *

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.

For Research Use Only. Not for use in diagnostic procedures.

Spot an opportunity for improvement?Share a Content Correction