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Lonza™ P3 Primary Cell 96-well Nucleofector™ Kit
For medium-throughput transfection of primary cells, e.g., human T cells, CD34 or rat neurons, when working with the 96-well Shuttle™ Add-On.
Brand: Lonza™ V4SP-3096
This item is not returnable.
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Description
- High transfection efficiencies for primary cells.
- Compatible with liquid handling systems for automated high-throughput electroporation.
- Conditions are transferable between 4D-Nucleofector™ System, 96-well Shuttle™ System and 384-well Nucleofector™ System.
- Applications:
- Transfection of low cell numbers in the range of 5 x 104 to 1 x 106 cells.
- High-throughput applications, e.g., screens of arrayed cDNA, RNAi or CRISPR libraries.
- This kit has been determined to be optimal for transfecting following cell types:
- B cell (human)
- CD34+ cell (human)
- Chondrocyte (human)
- DC (human)
- DC (mouse), mature
- ES (human), H9
- ES (mouse)
- Hepatocyte (human)
- HMEC (human)
- Keratinocyte (human)
- Macrophage (human)
- Monocyte (human)
- Neuron (rat), brain Hi/Cx
- NHBE (human)
- RPE (human)
- T cell (human), stim.
- T cell (mouse), unstim..
Specifications
| Clear | |
| 96-well Shuttle™ Add-On | |
| P3 Primary Cell Nucleofector™ Solution, Supplement 1, pmaxGFP™ Vector (1μg/μL in 10mM Tris, pH 8.0; 50 μg), 96-well Nucleocuvette™ Plate | |
| 7.1 to 7.3 |
| pmaxGFP™ | |
| Medium-throughput primary cell transfection | |
| 96 | |
| 2.25 mL P3 Primary Cell Nucleofector™ Solution, 0.5 mL Supplement 1, 50 μg pmax GFP™ Vector (1 μg/μL in 10 mM Tris pH 8.0), 1 x 96-well Nucleocuvette™ Plate (20 μL) |