ELISA
Brand: Invitrogen™ KHT0601
The Human Vascular Cell Adhesion Molecule 1 (Hu VCAM-1) ELISA quantitates Hu VCAM-1 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu VCAM-1. Principle of the method The Human VCAM-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine activated endothelium. The protein has a number of functions including the regulation of leukocyte migration, leukocyte-endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases.
| P19320 | |
| <0.5 ng/mL | |
| ELISA | |
| Human | |
| Colorimetric Microplate Reader | |
| CD106,INCAM-100 | |
| 6.4% | |
| Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers | |
| VCAM-1 | |
| RUO | |
| 2°C to 8°C | |
| 1 hrs 20 mins |
| 0.59 to 75 ng/mL | |
| HRP | |
| Plasma, Serum, Supernatant | |
| Colorimetric Detection | |
| 7412 | |
| 3 hr. | |
| 6.7 | |
| HRP | |
| 96 Tests | |
| Plasma 10 μL, Serum 10 μL, Supernatant 10 μL | |
| VCAM-1 (CD106) | |
| 3 hrs |
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A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.
| Lot Number | Certificate Type | Date | Catalog Number |
|---|---|---|---|
| 432307-002 | Certificate of Analysis | 24/03/2025 |
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