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Invitrogen™ Ni-NTA Agarose
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Quantity:
10 mL
25 mL
100 mL
Unit Size:
100mL
10mL
25mL
Description
Includes
The Ni-NTA resin is pre-charged and able to bind up to 50mg of recombinant protein per 1mL of resin. It is provided as a 50% slurry in 30% ethanol. The resin will appear blue in color when charged with Ni2+
- Ni-NTA Agarose is a nickel-charged affinity resin that can be used to purify recombinant proteins containing a polyhistidine (6xHis) sequence
- Proteins bound to the resin may be eluted with either low pH buffer or by competition with imidazole or histidine
- One-step purification can be performed under both native and denaturing conditions
- Ni-NTA uses the chelating ligand nitrilotriacetic acid (NTA) coupled to a cross-linked 6% agarose resin that is suitable for use in batch and gravity flow applications
- Guaranteed stable for 6 months when properly stored at 4°C

Specifications
Specifications
Content And Storage | The Ni-NTA resin is pre-charged and able to bind up to 50 mg of recombinant protein per 1 ml of resin. It is provided as a 50% slurry in 30% ethanol. The resin will appear blue in color when charged with Ni2+ . Store at +4°C. Ni-NTA resin is guaranteed stable for 6 months when properly stored. |
Stationary Phase | Ni-NTA |
Type | Agarose |
Quantity | 10 mL |
Form | Suspension |
Column Type | Affinity Column |
For Research Use Only. Not for use in diagnostic procedures.
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