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Invitrogen™ Human IgG Subclass ELISA Kit

ELISA

Brand: Invitrogen™ 991000

Product Code. 10455273

9910.00 NOK / 192 tests

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Description

The Human IgG Subclass Profile (Hu IgG) ELISA quantitates Hu IgG subclasses in human serum or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu igG1, IgG2, IgG3 or IgG4.

Principle of the method

The Human IL-10 US solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Human IgG, also known as human immunoglobulin G, is an antibody that is produced by the immune system in response to antigens. It is the most abundant immunoglobulin in the blood and plays a key role in defense against pathogens. IgG works by binding to specific antigens and promoting their clearance from the body through various mechanisms, including phagocytosis and complement activation. IgG is also involved in other immune responses, such as antibody-dependent cellular cytotoxicity and neutralization of toxins. Human IgG can be used in research to study the immune response to pathogens and to develop diagnostic tests and therapeutic interventions for infectious and autoimmune diseases.

Specifications

Conjugate	HRP
Sample Type	Serum
For Use With (Application)	Colorimetric Detection
Kit Contents	Pre-coated 96 well plates, Standard, Sample Diluent, Assay Buffer concentrate, Serum Control, MAbs to IgG Subclasses(4), Peroxidase-conjugated Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Marker Gene	IgG1, IgG2, IgG3, IgG4
Regulatory Status	RUO
Storage Requirements	2°C to 8°C
Test Time	20 mins

Product Type	ELISA
Target Species	Human
For Use With (Equipment)	Colorimetric Microplate Reader
Label or Dye	HRP
Quantity	2 x 96 Tests
Sample Volume	Serum 10 μL
Target	Human IgG
Total Assay Time	1 hrs 10 mins

Product Suggestions Safety and Handling

Safety and Handling

Product Identifier

Human IgG Subclass ELISA Kit

Signal Word

Warning

Hazard Category

Skin sensitiser Category 1

Hazard Statement

H317-May cause an allergic skin reaction.

Precautionary Statement

P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
P272-Contaminated work clothing should not be allowed out of the workplace.
P280-Wear protective gloves/protective clothing/eye protection/face protection.
P302+P352-IF ON SKIN: Wash with plenty of water/soap.
P333+P313-If skin irritation or rash occurs: Get medical advice/attention.
P362+P364-Take off contaminated clothing and wash it before reuse.
P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

MIXTURE LIST-Contains : 2-methyl-3(2H)-isothiazolone

Documents

Product Information

Manuals

Product Sheet: Human IgG Subclass Profile Version: 2.0 File size: 103.5 kB

Technical Data Sheet: Human IgG Subclass Profile, Lot No. 1694083 Version: MAN0014809 Rev. 1.0 (08Jan2016) File size: 219.8 kB

991000-Lot 766617 Human IgG ELISA Kit Version: 03/10 File size: 94.1 kB

991000_Lot_844404.pdf Version: 10/10 File size: 94.1 kB

Certificates

A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.

Lot Number

4 results found

Lot Number	Certificate Type	Date	Catalog Number
Lot Number439689-002	Certificate TypeCertificate of Analysis	Date16/06/2025	Catalog Number
Lot Number423936-001	Certificate TypeCertificate of Analysis	Date14/06/2025	Catalog Number
Lot Number439689-001	Certificate TypeCertificate of Analysis	Date03/04/2025	Catalog Number
Lot Number439689-001	Certificate TypeCertificate of Analysis	Date10/02/2025	Catalog Number

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