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Cytiva Ni Sepharose™ excel

Ni Sepharose excel affinity media for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).

Brand:  Cytiva 17371201


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Regulatory: This product contains a substance listed in Annex XIV to REACH Regulation (EC) No 1907/2006, all customers must complete a declaration before delivery.

Product Code. 13415187

  • 5225.00 NOK / 25mL
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Description

Description

  • Load eukaryotic cell culture samples containing secreted histidine-tagged proteins directly with retained binding capacity.
  • Increase target protein yield and decrease degradation through reduced and simplified sample handling.
  • Choose between several formats for screening and preparative purification of histidine-tagged proteins.
  • Ni Sepharose excel is an IMAC medium precharged with nickel ions very strongly bound to a chelating ligand, allowing loading of samples that usually cause stripping of metal ions.
  • Ni Sepharose excel is designed primarily for capture and purification of histidine-tagged proteins secreted into cell culture supernatants from eukaryotic cells such as insect cells or CHO cells.

TRUSTED_SUSTAINABILITY
Specifications

Specifications

0.01M HCl and 0.01M NaOH (one week), 10mM EDTA, 5mM DTT, 1M NaOH, 5mM TCEP, 20mM beta-Mercapto ethanol and 6M Guanidine-HCl (24 Hours), 500mM Imidazole and 100mM EDTA (2 Hours), 30% 2-Propanol
25 mL
2 to 12
6% Highly Cross-linked Agarose
Capture and purification of His-tagged Proteins secreted into eukaryotic cell culture supernatants by immobilized Metal ion affinity chromatography (IMAC)
Ni Sepharose excel chromato graphy media
150 to 600cm/hr.
90μm
4° to 30°C
Ni-Sepharose
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Safety and Handling

Safety and Handling

Signal Word
  • ||Warning

Hazard Category
  • Flammable liquid Category 3

Hazard Statement
  • H226-Flammable liquid and vapour.
  • EUH208-Contains Nickel. May produce an allergic reaction.

Precautionary Statement
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information
  • MIXTURE LIST-Contains : ethanol
  • UNKNOWN ACUTE-16.5 per cent of the mixture consists of component(s) of unknown acute toxicity.

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