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Cytiva Ni Sepharose™ excel
Ni Sepharose excel affinity media for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).
Brand: Cytiva 17-3712-02
This item is not returnable.
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Description
Ni Sepharose excel enables direct loading of samples without having to perform extensive and time-consuming pretreatment procedures.
- Load eukaryotic cell culture samples containing secreted histidine-tagged proteins directly with retained binding capacity.
- Increase target protein yield and decrease degradation through reduced and simplified sample handling.
- Choose between several formats for screening and preparative purification of histidine-tagged proteins.
- Ni Sepharose excel is an IMAC medium precharged with nickel ions very strongly bound to a chelating ligand, allowing loading of samples that usually cause stripping of metal ions.
- Ni Sepharose excel is designed primarily for capture and purification of histidine-tagged proteins secreted into cell culture supernatants from eukaryotic cells such as insect cells or CHO cells.
Specifications
| 0.01M HCl and 0.01M NaOH (one week), 10mM EDTA, 5mM DTT, 1M NaOH, 5mM TCEP, 20mM beta-Mercapto ethanol and 6M Guanidine-HCl (24 Hours), 500mM Imidazole and 100mM EDTA (2 Hours), 30% 2-Propanol | |
| 100mL | |
| 2 to 12 | |
| 6% Highly Cross-linked Agarose | |
| Capture and purification of His-tagged Proteins secreted into eukaryotic cell culture supernatants by immobilized Metal ion affinity chromato graphy (IMAC) |
| Ni Sepharose excel chromato graphy media | |
| 150 to 600cm/hr. | |
| 90μm | |
| 4° to 30°C | |
| Ni-Sepharose |
Safety and Handling
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Warning
Hazard Category
- Flammable liquid Category 3
Hazard Statement
- H226-Flammable liquid and vapour.
- EUH208-Contains nickel. May produce an allergic reaction.
Precautionary Statement
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P235-Keep cool.
- P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : nickel, ethanol