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Cytiva Ni Sepharose™ excel

Product Code. 12621206 Shop All Cytiva Products
500 mL, 500mL
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Quantity:
25 mL
100 mL
500 mL
Unit Size:
100mL
25mL
500mL
This item is not returnable. View return policy
This item is not returnable. View return policy

Ni Sepharose excel affinity media for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).

Ni Sepharose excel enables direct loading of samples without having to perform extensive and time-consuming pretreatment procedures.

  • Load eukaryotic cell culture samples containing secreted histidine-tagged proteins directly with retained binding capacity.
  • Increase target protein yield and decrease degradation through reduced and simplified sample handling.
  • Choose between several formats for screening and preparative purification of histidine-tagged proteins.
  • Ni Sepharose excel is an IMAC medium precharged with nickel ions very strongly bound to a chelating ligand, allowing loading of samples that usually cause stripping of metal ions.
  • Ni Sepharose excel is designed primarily for capture and purification of histidine-tagged proteins secreted into cell culture supernatants from eukaryotic cells such as insect cells or CHO cells.

TRUSTED_SUSTAINABILITY

Specifications

Buffer 0.01M HCl and 0.01M NaOH (one week), 10mM EDTA, 5mM DTT, 1M NaOH, 5mM TCEP, 20mM beta-Mercapto ethanol and 6M Guanidine-HCl (24 Hours), 500mM Imidazole and 100mM EDTA (2 Hours), 30% 2-Propanol
Product Type Ni Sepharose excel chromato graphy media
Quantity 500 mL
Flow Rate 150 to 600cm/hr.
pH Range 2 to 12
Particle Size 90μm
Support Type 6% Highly Cross-linked Agarose
Content And Storage 4° to 30°C
For Use With (Application) Capture and purification of His-tagged Proteins secreted into eukaryotic cell culture supernatants by immobilized Metal ion affinity chromato graphy (IMAC)
Matrix Ni-Sepharose
compliance-icons
Signal Word
  • Warning
Hazard Category
  • Flammable liquid Category 3
Hazard Statement
  • H226-Flammable liquid and vapour.
  • EUH208-Contains . May produce an allergic reaction.
Precautionary Statement
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P235-Keep cool.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
  • MIXTURE LIST-Contains : Ethanol, Nickel
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