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Cytiva Ni Sepharose™ excel
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Quantity:
25 mL
100 mL
500 mL
Unit Size:
100mL
25mL
500mL
Description
Ni Sepharose excel enables direct loading of samples without having to perform extensive and time-consuming pretreatment procedures.
- Load eukaryotic cell culture samples containing secreted histidine-tagged proteins directly with retained binding capacity.
- Increase target protein yield and decrease degradation through reduced and simplified sample handling.
- Choose between several formats for screening and preparative purification of histidine-tagged proteins.
- Ni Sepharose excel is an IMAC medium precharged with nickel ions very strongly bound to a chelating ligand, allowing loading of samples that usually cause stripping of metal ions.
- Ni Sepharose excel is designed primarily for capture and purification of histidine-tagged proteins secreted into cell culture supernatants from eukaryotic cells such as insect cells or CHO cells.

Specifications
Specifications
Buffer | 0.01M HCl and 0.01M NaOH (one week), 10mM EDTA, 5mM DTT, 1M NaOH, 5mM TCEP, 20mM beta-Mercapto ethanol and 6M Guanidine-HCl (24 Hours), 500mM Imidazole and 100mM EDTA (2 Hours), 30% 2-Propanol |
Product Type | Ni Sepharose excel chromato graphy media |
Quantity | 500 mL |
Flow Rate | 150 to 600cm/hr. |
pH Range | 2 to 12 |
Particle Size | 90μm |
Support Type | 6% Highly Cross-linked Agarose |
Content And Storage | 4° to 30°C |
For Use With (Application) | Capture and purification of His-tagged Proteins secreted into eukaryotic cell culture supernatants by immobilized Metal ion affinity chromato graphy (IMAC) |
Matrix | Ni-Sepharose |
Safety and Handling

Signal Word
- Warning
- Flammable liquid Category 3
- H226-Flammable liquid and vapour.
- EUH208-Contains . May produce an allergic reaction.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P235-Keep cool.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contains : Ethanol, Nickel
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