MP Biomedicals™ FastDNA™-96 Plant/Seed DNA Kit

Beschreibung

• Product Overview: Rapid, High-Throughput Isolation of PCR-Ready Genomic DNA from Plant and Seed Samples The FastDNA™-96 Plant and Seed DNA Kit is designed for the simple and rapid isolation of PCR-quality DNA from vegetative samples in approximately 50 minutes. Plant samples including stems, roots, leaves, buds, flowers, fruits and seeds are efficiently lysed in approximately 60 seconds with the FastPrep-96™ Instrument. Up to 80 mg samples are placed into each 1.2 mL tube of a FastDNA-96™ Lysing Matrix Rack (96-deep well plate) containing 2.0 mm specially stabilized Zirconium oxide lysing particles, which are designed to efficiently lyse a wide variety of plant specimens, while in the presence of a specially formulated lysis solution. The cleared lysate is then applied to a binding matrix to isolate the DNA, and spin column plates are used to simplify liquid handling. Eluted DNA is then applied to the MP-96 Inhibitor Removal Plate which removes tannins, polyphenols and polysaccharides that can inhibit PCR. Purified DNA is eluted in an EDTA-free, DNA elution solution, and is ready for downstream applications including digestion, electrophoresis, arrays and PCR (A260/A280 ratios ≥1.8). Yield is typically 5 μg of total DNA eluted in 50 to 100 μL of elution solution. This procedure can be performed in minutes, and there is no need for organic denaturants or proteinases.
• Applications: The FastDNA™-96 Plant/Seed DNA Kit for FastPrep™-96 is used for the simple, rapid isolation of inhibitor-free, PCR-quality DNA from a variety of plant sample sources including leaves, stems, buds, flowers, fruit, and seeds. Eluted DNA can be used for downstream molecular-based applications including PCR, arrays, digestion, electrophoresis etc.
• Sample Source: Plant Tissues
• Sample Type: Seeds, Plants, Roots, Buds, Flowers, Fruits, Leaves, Stems
• Sample Volume: Up to 80 mg