Invitrogen™ Mouse MECP2 (aa 469-486) Synthetic Peptide

Description

Mouse MeCP2 (residues 469-486) Synthetic Peptide, PEP-121, from Invitrogen.

MECP2 belongs to a family of nuclear proteins (including MBD1, MBD2, MBD3 and MBD4) that have a methyl-CpG binding domain. MECP2 is capable of binding specifically to methylated DNA. DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. MECP2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. PEP-121 is a 18 amino acid synthetic peptide whose sequences are derived from amino acids 469-486 of mouse MeCP2 protein with a amino-terminal cysteine added for carrier protein conjugation. The sequence of this peptide is (amino to carboxy terminus): C-P (469)-N-R-E-E-P-V-D-S-R-T-P-V-T-E-R-V-S (486)PEP-121 may be used for neutralization and control experiments with the polyclonal antibody that reacts with this product and mouse MeCP2, catalog &Num; PA1-888. Using a solution of peptide of equal volume and concentration to the corresponding antibody will yield a large molar excess of peptide (∽70-fold) for competitive inhibition of antibody-protein binding reactions.Reconstitute with 0.1 mL of distilled water.