Invitrogen™ Qtracker™ 525 Cell Labeling Kit

Description

• Use proprietary targeting peptide to deliver green-fluorescent Qdot 525 nanocrystals into cytoplasm of live cells
• Once inside cells, Qtracker labels provide intense, stable fluorescence that can be traced through several generations, and are not transferred to adjacent cells in population
• Qtracker 525 label has Ex/Em (405-485/525)nm
• Provide intense, stable fluorescence that can be traced through several generations
• Excellent tools for long-term tracking or imaging studies of live cells, including migration, motility, morphology, and other cell function assays
• Cytoplasmic delivery by this mechanism is not mediated by specific enzyme; therefore, no cell-type specificity has been observed
• Delivery is typically accomplished in less than 1 hour

• Compatible with serum-sensitive cells; intense fluorescence is maintained in complex cellular environments and under various biological conditions including changes in intracellular pH, temperature, and metabolic activity
• Observe labeled cells using extensive continuous illumination, without photobleaching and degradation problems often associated with organic dyes
• Distributed in vesicles in cytoplasm, and are inherited by daughter cells for at least six generations
• Can be seen up to a week after delivery in some cell lines
• Do not leak out of intact cells to be taken up by adjacent cells in population
• Qtracker reagent-labeled live cells can be easily monitored on variety of platforms, including flow cytometry, fluorescence/confocal microscopy, fluorescence microplate readers, and high-content imaging systems
• Cytotoxicity of materials has been tested in variety of cell lines including CHO, HeLa, U-118, 3T3, HUVEC, and Jurkat cells: Labeling appears to exert minimal impact on cellular surface marker expression, cell proliferation, cellular enzyme activity, and cell motility
• Post-labeling, researchers have demonstrated wide variety of applications including cell co-culture and cell assembly into heterotypic assemblies, multilineage differentiation, trans-differentiation versus cell fusion, embryonic and mesenchymal stem cell tracking, and cell migration dynamics

Cell Analysis, Cell Structure, Cell Tracing and Tracking, Cellular Imaging, Cytoplasm and Cytosol, Immunofluorescence (IF), Organelle Tracing

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