Invitrogen™ NucBlue™ Live ReadyProbes™ Reagent (Hoechst 33342)

Description

Hoechst 33342 is perhaps the most ubiquitous nuclear fluorescence stain for live and fixed cells and tissue sections. The cell permeable stain binds preferentially to AT regions of DNA. It is excited by UV light and emits blue fluorescence at 460nm when bound to DNA.

• Ready-to-use liquid formulation of high-purity Hoechst 33342
• Typically Hoechst 33342 is supplied as solid or highly concentrated solution that requires weighing out and diluting several thousand-fold before use, and recommended storage is generally in freezer or refrigerator
• This version is ready to use and can be stored next to a microscope or workstation. A liquid formulation in ultra-convenient dropper bottle allows you to see
• Spectral properties of Hoechst 33342 (2'-[4-ethoxyphenyl]-5-[4-methyl-1-piperazinyl]-2,5'-bi-1H-benzimidazole), including a large Stokes shift, make it ideal for use with green (Alexa Fluor 488, FITC, GFP) and red (Alexa Fluor 594, Texas Red, rhodamine, mCherry, mKate-2) fluorophores in multicolor experiments
• Because of its high affinity to DNA, Hoechst 33342 is also frequently used in cell counting, cell cycle, and cell replication studies to distinguish condensed nuclei in apoptotic cells, for cell-cycle studies, as a nuclear segmentation tool in high content imaging analysis, and to sort cells based on their DNA content
• May be added directly to cells in full media, or buffer solutions
• In most cases 2 drops/mL and incubation of 15 to 30 minutes will give bright nuclear staining; however, optimization may be needed for some cell types, conditions, and applications
• In most cases, staining intensity increases with time if cells are not washed prior to imaging.
• Excited by UV light at 360nm when bound to DNA, with an emission maximum at 460nm
• Detected through blue/cyan filter

Apoptosis, Cell Analysis, Cell Cycle, Cell Proliferation, Cell Structure, Cell Tracing and Tracking, Cell Viability and Cytotoxicity, Cell Viability, Proliferation and Function, Cellular Imaging, DNA Fragmentation, Flow Cytometry, Flow Cytometry Staining by Cell Structure, Immunofluorescence (IF), Immunofluorescence Counterstaining, Mounting and Fade Prevention, Immunofluorescence Staining and Detection, Nucleus, Nucleoli and Nuclear Envelope, Organelle Tracing

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Shipping Condition: Room temperature