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Invitrogen™ CellLight™ Late Endosomes-RFP, BacMam 2.0

Provides easy way to label late endosomes with red fluorescent protein in live cells

Brand:  Invitrogen™ C10589

3920.00 NOK valid until 2025-06-30
Use promo code "24074" to get your promotional price.


Product Code. 11682149

  • 5600.00 NOK / 1mL

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Description

Description

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses RFP fused to Rab7a. You can observe late endosomes-RFP behavior in live cells independently of organelle pH and label with multiple tracking or tracing dyes to image dynamic cellular processes.

Cells expressing CellLight constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

BacMam Technology

CellLight Late Endosomes-RFP, BacMam 2.0, is a fusion construct of Rab7a and TagRFP, providing accurate and specific targeting to cellular Late Endosomes-RFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

  • Fast and convenient: simply add CellLight reagent to cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
  • Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
  • Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
  • Less toxic: CellLight reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

Cell Analysis, Cell Structure, Cell Tracing & Tracking, Cellular Imaging, Endosomes, Immunofluorescence (IF), Organelle Tracing

Order Info

Shipping Condition: Wet Ice

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Red-Orange
RFP (TagRFP)
1 mL
Visible
CellLight™, Molecular Probes™
Endocytosis
Fluorescent Dye
Endosomes
Store at 2°C to 6°C, protected from light. Do Not Freeze.
Fluorescence Microscope, Flow Cytometer
Fluorescence
Liquid
Wet Ice
Fluorescence Intensity
Endosomes Label
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5 results found
Lot Number Certificate Type Date Catalog Number
Lot Number2816110 Certificate TypeCertificate of Analysis Date20/03/2024 Catalog NumberC10589
Lot Number2720256 Certificate TypeCertificate of Analysis Date12/08/2023 Catalog NumberC10589
Lot Number2515913 Certificate TypeCertificate of Analysis Date28/10/2022 Catalog NumberC10589
Lot Number2373221 Certificate TypeCertificate of Analysis Date07/09/2021 Catalog NumberC10589
Lot Number2289870 Certificate TypeCertificate of Analysis Date03/06/2021 Catalog NumberC10589
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Product Title
Invitrogen™ CellLight™ Late Endosomes-RFP, BacMam 2.0 > 1mL

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For Research Use Only. Not for use in diagnostic procedures.