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  5. Invitrogen™ mMESSAGE mMACHINE™ T3 Transcription Kit
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Invitrogen™ mMESSAGE mMACHINE™ T3 Transcription Kit

Obtain 10–50 times yield of conventional in vitro transcription reactions

Brand:  Invitrogen™ AM1348

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Product Code. 10665605

  • 6520.00 NOK / 25 reactions

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Description

Description

Ambion mMESSAGE mMACHINE High Yield Capped RNA Transcription Kits use Ambion's patented high-yield transcription technology for the routine synthesis of 15 - 35μg of 7-methyl guanosine capped RNA from 1μg of template DNA. Each kit includes sufficient reagents for 25 reactions.

  • High yields of capped RNA—up to 39μg of RNA per reaction
  • Extra convenience—cap analog-nucleotide mix provided
  • Simplified reaction format
  • Synthesized transcripts ideal for microinjection, in vitro translation, or transfection in a 20μL reaction during a 2 hour incubation, mMESSAGE mMACHINE High Yield Capped RNA Transcription will yield up to 35μg of capped RNA
  • ™
  • 10–50 times yield obtained with conventional in vitro transcription reactions
  • Ratio of cap analog to GTP has been optimized to allow compromise between yield (15 - 35μg) and proportion of transcripts that are capped (80%)
  • Contain a LiCl precipitation solution, efficient for separating proteins and unincorporated nucleotides (including free cap analog) from the capped RNA, allowing an increased efficiency of translation
  • Optimized for use with polymerases included in kit
  • Contains 57U of cap analog

TRUSTED_SUSTAINABILITY
Specifications

Specifications

T3 Enzyme Mix, 10X Reaction Buffer 2XNTP⁄CAP Solution, GTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
Kit
In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
Ambion™, mMESSAGE mMACHINE™
25 Reactions
Dry Ice
PCR Products, Linearized DNA plasmid
Synthetic mRNA, mRNA
mRNA, Synthetic mRNA
25 Reactions
T3 In Vitro Transcription kit
T3
20-30 μg
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