Applied Biosystems™ MagMAX™ Cell-Free DNA Isolation Kit

Description

The MagMAX™ Cell-Free DNA Isolation Kit is designed for enrichment of circulating cell-free DNA (cfDNA) and optimized for use with biological samples such as serum and plasma. The kit is based on MagMAX™ magnetic-bead technology, enabling reproducible recovery of high-quality DNA that is suitable for a broad range of applications, including real-time PCR, digital PCR, and next-generation sequencing.

Scalable isolation of circulating nucleic acid from plasma, serum, and urine
cfDNA recovery is challenging due to the limited amount present in cell-free samples. The MagMAX Cell-Free DNA Isolation Kit addresses this with its magnetic bead-based purification format which allows you to easily process a wide range of sample input volumes, from 100 μL to 10 mL. When used with either a KingFisher™ Duo Prime or KingFisher™ Flex magnetic particle processor, 6 to 24 plasma/serum samples of 2 mL input volume can be isolated at once. Alternatively, samples can be processed manually with the use of a magnetic stand.

Features of the MagMAX Cell-Free DNA Isolation Kit include:
• Flexible design that permits both manual and automated isolation of cfDNA
• Scalable format that allows for higher and lower input volumes
• Automation-ready, phenol-free extraction
• Fast procedure, which allows 6 to 24 samples to be processed typically in 40 minutes or less when used with the KingFisher Duo Prime Magnetic Particle Processor or the KingFisher Flex Magnetic Particle Processor with a 24 Deep-Well Head
• Wide sample volume inputs range from 500 μL to 10 mL of plasma/serum or urine
• Contains Dynabeads™ MyOne™ Silane for consistent isolation of cfDNA
• Elution volumes range from 15 μL to 50 μL

Unlock a wealth of information with cell-free DNA
Isolating and analyzing circulating cfDNA from plasma, serum, or urine has the potential to serve as a non-invasive approach to detect and monitor targets associated with certain disease states such as cancer. The first step in attaining this valuable information is efficient nucleic acid isolation that specifically recovers the circulating cfDNA while leaving the larger DNA molecules behind. This helps ensure that the shorter, fragmented DNA is concentrated and ready for downstream analysis using real-time PCR, digital PCR, or next-generation sequencing.

Benefits of MagMAX magnetic bead–based purification
Magnetic beads offer many benefits compared to other technologies for isolating cfDNA. Beads bind the DNA more efficiently than glass fiber filters, resulting in higher and more consistent yields. Additionally, because filters and vacuum manifolds are not used, there is no risk of cellular particulates clogging these items during the extraction process. This clogging issue is of particular concern with protein-rich, large-volume samples such as plasma that are commonly used in cfDNA studies.

Optimized surface chemistry
The Dynabeads MyOne Silane contained in the MagMAX Cell-Free DNA Isolation Kit have an optimized silica-like surface chemistry with high specific surface area that allows efficient kinetics and high sensitivity in nucleic acid capture. These aspects make Dynabeads MyOne Silane ideal for the recovery of circulating cfDNA targets present at low concentrations in cell-free body fluids. Once captured, these rare DNA fragments can then be eluted in small elution volumes of 15 μL to 50 μL and are ready for downstream applications.