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Invitrogen™ Lipofectamine™ 3000 Transfection Reagent

Lipofectamine™ 3000 Transfection Reagent leverages our most advanced lipid nanoparticle technology to provide superior transfection performance with improved application outcomes and reproducible results.

Brand: Invitrogen™ L3000008

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Product Code. 15292465

6250.00 NOK / 0.75mL

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Description

This reagent delivers superior transfection efficiency and improved cell viability for the widest range of hard-to-transfect and common cells (e.g., HEK293, HeLa).

Successfully transfect the widest variety of biologically relevant cell types with a reagent that offers:

Superior performance—our highest efficiency reagent for difficult-to-transfect cells
Improved cell viability—gentle on your cells, with low toxicity
Versatile—one reagent for DNA, RNA, and co-transfection

Efficiently Transfect Difficult-to-Transfect Cells
Lipofectamine 3000 reagent is designed to efficiently transfect difficult-to-transfect cells, yielding superior transfection performance across the broadest array of cell types. Successfully transfected cell types include:

Fibroblast (3T3, COS-7)
Myoblast (C2C12, L6 CRL-1458)
Hepatocyte (HepG2, HuH7)
Erythroleukemic cell (K562)
Breast carcinoma (MCF7, Hs578T)
Prostate carcinoma (LNCap)
Lung carcinoma (A549, NCI-H460)
Osteosarcoma (U-2 OS, Saos-2)
Colon carcinoma (Caco2, SW480)
Pancreatic carcinoma (PANC-1)
Skin melanoma (SK-MEL-28)

Transfect Common Cells Efficiently and Cost-Effectively
For common cell types, Lipofectamine 3000 reagent is more cost-effective than other reagents due to its high concentration and low usage amount. The 1.5mL size provides up to 1500 transfection reactions (in 24-well plates).

Achieve Superior Transfection Efficiency While Maintaining Low Toxicity
Lipofectamine 3000 reagent maintains a high transfection efficiency within a robust dynamic range of lipid doses for quick and easy optimization. A low toxicity lipid dose (0.75μL for 24-well plates) is suggested for applications requiring minimal disruption of the cells.

Enable High Transfection Efficiency in Novel Genome Editing Applications
Lipofectamine 3000 reagent is suitable for novel genome editing applications. It increases the likelihood of successful cleavage and recombination with TALENs or CRISPRs through highly efficient transfection, ultimately maximizing the efficiency of genetic modifications and simplifying the downstream processes.

Specifications

Classification	Animal Origin-Free
Cell Type	Established Cell Lines, Stem Cells, Primary Cells, Hard-to-Transfect Cells
Sample Type	Plasmid DNA, Synthetic siRNA, RNAi Plasmids (shRNA, miR)
For Use With (Application)	Transfection
Product Line	Lipofectamine™
Quantity	0.75 mL

Content And Storage	Contains one vial of Lipofectamine™ 3000 Reagent and one vial of P3000TM Enhancer Reagent. Store at 2–8°C. Do not freeze.
Format	6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
Transfection Technique	Lipid-based Transfection
Green Features	Sustainable packaging
Product Type	Transfection Reagent
Serum Compatible	Yes

Product Suggestions Documents

Documents

Product Information

Methods & Protocols

Protocol: Lipofectamine 3000 Transfection Reagent NCI-H358 Lung cancer cells File size: 695.1 kB

Protocol: Lipofectamine 3000 Transfection Reagent Calu-3 Lung cancer cells File size: 1.1 MB

Protocol: Lipofectamine 3000 Transfection Reagent NCI-H1975 Lung cancer cells File size: 1 MB

Protocol: Calu-1 lung cancer cell transfection using Lipofectamine 3000 File size: 126.5 kB

Protocol: Hepa-1-6 liver cancer cell transfection using Lipofectamine 3000 File size: 130.9 kB

Protocol: MDA-MB-453 breast cancer cell transfection using Lipofectamine 3000 File size: 135.6 kB

Protocol: V79 HG04 lung cancer cell transfection using Lipofectamine 3000 File size: 118.9 kB

Protocol: NCI-H1650 lung cancer cell transfection using Lipofectamine 3000 File size: 115.8 kB

Protocol: NCI-H522 lung cancer cell transfection using Lipofectamine 3000 File size: 117.2 kB

Protocol: NCI-H520 lung cancer cell transfection using Lipofectamine 3000 File size: 112 kB

Manuals

Lipofectamine 3000 Reagent Scaling Table File size: 36.5 kB

Lipofectamine 3000 Reagent Scaling (Chinese) Version: MAN0009872 B.0 (3 Feb 14) File size: 87.9 kB

Scientific Resources

Reference Materials

Lipofectamine 2000, 3000, MessengerMAX, and RNAiMAX Transfection Reagents Green Fact Sheet File size: 278.5 kB

Protocol: Lipofectamine 3000 Transfection Reagent PC-3 Prostate cancer cells File size: 1.1 MB

Protocol: Lipofectamine 3000 Transfection Reagent H-4-II-E Liver cancer cells File size: 1 MB

Protocol: Lipofectamine 3000 Transfection Reagent Fao Liver cancer cells File size: 1 MB

Protocol: Lipofectamine 3000 Transfection Reagent PLC/PRF/5 Liver cancer cells File size: 1.3 MB

Protocol: Lipofectamine 3000 Transfection Reagent T-47D Breast cancer cells File size: 1.1 MB

Protocol: Lipofectamine 3000 Transfection Reagent C4 Prostate cancer cells File size: 1.3 MB

Protocol: Lipofectamine 3000 Transfection Reagent ZR-75-1 Breast cancer cells File size: 1.1 MB

Protocol: Lipofectamine 3000 Transfection Reagent SK-HEP-1 Liver cancer cells File size: 1.1 MB

Protocol: Lipofectamine 3000 Transfection Reagent MDA-MB-468 Breast cancer cells File size: 985.4 kB

Brochures

Protocol: D17 lung cancer cell transfection using Lipofectamine 3000 File size: 117.1 kB

Certificates

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Lot Number

5 results found

Lot Number	Certificate Type	Date	Catalog Number
Lot Number3039409	Certificate TypeCertificate of Analysis	Date31/01/2025	Catalog NumberL3000008
Lot Number2471954	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog NumberL3000008
Lot NumberCN2513605	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog NumberL3000008
Lot Number2489212	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog NumberL3000008
Lot NumberCN2541162	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog NumberL3000008

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