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Isoamyl Alcohol, MP Biomedicals™
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Quantity:
100 mL
500 mL
1 L
Description
Isoamy alcohol is routinely used in molecular biology, especially in the purification of DNA. Isoamyl alcohol is also used in the extraction of ethidium bromide from DNA solutions and in the radiolabeling of RNA transcripts in nuclei that have been isolated from tissue.
- Widely used in conjunction with phenol and chloroform (25:24:1—phenol:chloroform:isoamyl alcohol) for the removal of proteins from the nucleic acid solutions by extraction
- Isoamyl alcohol is added to the phenol solution to help inhibit RNase activity and to help prevent the solubilization in the phenol phase of long RNA molecules with long poly(A) portions
- It will also help in reducing the foaming during the extraction process
Isoamy alcohol has various miscellaneous uses, including:
- HPLC analysis of various pharmaceuticals and metabolites
- Analysis of oxidized and reduced pyridine nucleotides and adenylates in organic phenol extracts from mitochondria
- Isolation of mRNA from a thermophilic cyanobacterium
- Solvent for fats, resins and alkaloids
- Manufacturing of isoamyl (amyl) compounds, isovaleric acid, mercury fulminate, pyroxylin, artificial silk, lacquers and smokeless powders
- For dehydrating celloidin solutions
- For determining fat in milk
Specifications
Specifications
Melting Point | -117.2°C (literature) |
Density | 0.81g/mL (water=1) at 25°C (Lit.) |
Boiling Point | 131°C (literature) |
Refractive Index | n20/D 1.406 (Lit.) |
Quantity | 100 mL |
Solubility Information | Miscible with alcohol, ether, benzene, chloroform, petroleum ether, glacial acetic acid, oils. |
Vapor Density | 3.04 (air=1) (Lit.) |
Formula Weight | 88.15 |
Vapor Pressure | 2.4mmHg at 20°C, 2.37mmHg at 25°C (Lit.) |
Percent Purity | ≥99% |
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Safety and Handling
Product Identifier
- Isoamyl Alcohol
- Warning
- Acute toxicity Category 4
- Serious eye damage/eye irritation Category 2
- Flammable liquid Category 3
- Skin corrosion/irritation Category 2
- Specific target organ toxicity Category 3
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H332-Harmful if inhaled.
- H336-May cause drowsiness or dizziness.
- P101-If medical advice is needed, have product container or label at hand.
- P102-Keep out of reach of children.
- P103-Read label before use.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P405-Store locked up.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contains : Isoamyl Alcohol
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