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Invitrogen™ X-Gal
X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactoside) is a histochemical substrate for β-galactosidase. This substrate yields a blue precipitate upon hydrolysis, making it suitable for use in immunoblotting or immunocytochemical assays.
Brand: Invitrogen™ 15520018
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Description
X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactoside) is a histochemical substrate for β-galactosidase. This substrate yields a blue precipitate upon hydrolysis, making it suitable for use in immunoblotting or immunocytochemical assays. Bacteria containing active β-galactosidase produce blue colonies when grown on plates containing X-Gal. X-Gal is supplied as white crystals and is soluble in dimethylformamide.
Primary applications
- Blue-white screening—detect functional β-galactosidase (reporter gene expression)
- Histochemical detection of β-galactosidase activity in fixed tissues
- X-gal overlay assay
Find the media format that fits your needs
We offer many ready-to-use media formulations, such as LB Broth (10855001), Terrific Broth (A1374301) or S.O.C recovery medium (15544034).
We also offer IPTG (15529019) for blue-white screening.
Explore other media formats for bacterial growth.
Order Info
Approved for shipment at Room Temperature or on Wet Ice.

Specifications
5-bromo-4-chloro-3-indolyl-β-D-galactoside | |
Colorimetric | |
Bottle | |
X-Gal Substrate | |
Approved for shipment at Room Temperature or on Wet Ice |
• X-Gal, powder, 1 g | |
X-Gal, Cell-Permeant | |
Immunoblotting | |
1 g | |
Powder |
Certificates
A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.
Lot Number | Certificate Type | Date | Catalog Number |
---|---|---|---|
47510824 | Certificate of Analysis | 31/01/2025 | 15520018 |
L15110221 | Certificate of Analysis | 29/01/2025 | 15520018 |
16490219 | Certificate of Analysis | 29/01/2025 | 15520018 |
41990923 | Certificate of Analysis | 29/01/2025 | 15520018 |
36310423 | Certificate of Analysis | 29/01/2025 | 15520018 |
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For Research Use Only. Not for use in diagnostic procedures.