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Invitrogen™ Ribonuclease H

Specifically degrades RNA strand of RNA-DNA hybrid to produce 5' phosphate-terminated oligoribonucleotides and single-stranded DNA

Brand:  Invitrogen™ 18021014

1470.98 NOK valid until 2025-03-28
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Product Code. 10368002

  • 1896.00 NOK / Each

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Includes: Ribonuclease H containing 30 units supplied at a concentration of 2U/μL
This item is not returnable. View return policy

Description

Description

  • Source: Purified from E. coli expressing the E. coli RNase H gene on a plasmid
  • Performance and quality testing: Ribonuclease, nonspecific endodeoxyribonuclease, 3' and 5' exodeoxyribonuclease
  • Unit definition: One unit hydrolyzes 1nmol of RNA in 3H-labeled poly(A)poly(dT) to acid-soluble material in 20 min. at 37°C
  • Unit reaction conditions: 20mM Tris-HCl (pH 7.5), 0.1M KCl, 10mM MgCl2 , 0.1mM DTT, 5% (w/v) sucrose, 0.5nmol 3H-labeled poly(A)poly(dT), and enzyme in 50μL for 20 min. at 37°C

Removal of mRNA during second-strand cDNA synthesis; Removal of poly(A) sequences from mRNA in the presence of oligo(dT); Oligodeoxyribonucleotide-directed cleavage of RNA; PCR & Real-Time PCR; Reverse Transcription

Order Info

Shipping Conditions: Approved for shipment on wet or dry ice

TRUSTED_SUSTAINABILITY
Specifications

Specifications

2 U/μL
Approved for shipment on Wet or Dry Ice
30 U
1 vial of Ribonuclease H containing 30 units supplied at a concentration of 2U/μl

Store at -20°C in a non-frost-free freezer.
RNase
Ribonuclease H
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Product Sheet: Ribonuclease H Version: A.0 File size: 197.3 kB
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5 results found
Lot Number Certificate Type Date Catalog Number
Lot Number3041350 Certificate TypeCertificate of Analysis Date31/01/2025 Catalog Number18021014
Lot Number2553831 Certificate TypeCertificate of Analysis Date29/01/2025 Catalog Number18021014
Lot Number2822758 Certificate TypeCertificate of Analysis Date28/01/2025 Catalog Number18021014
Lot Number2878503 Certificate TypeCertificate of Analysis Date26/01/2025 Catalog Number18021014
Lot Number2978833 Certificate TypeCertificate of Analysis Date26/01/2025 Catalog Number18021014
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