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Invitrogen™ pRSET A, B, & C Bacterial Expression Vectors

Designed for high-level prokaryotic expression controlled by the strong bacteriophage T7 promoter

Brand:  Invitrogen™ V35120

Product Code. 10113802

  • 8950.00 NOK / 1 set

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Includes: 20μg of each pRSET A, B and C vector is provided lyophilized. TOP10F' E. coli stab, BL21(DE3)pLysS E. coli stab, and BL21 pRSET/lacZ control E. coli stab are also included.
This item is not returnable. View return policy

Description

Description

  • Expression is induced by production of T7 RNA polymerase in BL21(DE3) E. coli
  • These cells also produce T7 lysozyme to reduce basal expression of target genes
  • pRSET vector offers bacteriophage T7 promoter for high-level expression
  • T7 gene 10 sequence to provide protein stability
  • N-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating resin and detection with an Anti-HisG Antibody
  • N-terminal Xpress epitope for detection with the Anti-Xpress Antibody
  • Enterokinase cleavage site for removal of fusion tag
  • Each of three vectors provided (A, B, and C) has N-terminal tag coding sequence in a different reading frame relative to multiple cloning site to simplify in-frame cloning of the gene

Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Inducible
T7
None
Ampicillin (AmpR)
His Tag (6x)
20 μg
IPTG
Bacterial Expression Vector
20 μg of each pRSET A, B, & C vector is provided lyophilized. TOP10F´, BL21(DE3)pLysS, and BL21 pRSET/lacZ control E. coli stabs are also included. Store E. coli stabs at 2–8°C. All components are guaranteed stable for 6 months when properly stored.
EK (Enterokinase) Recognition Site
Restriction Enzyme/MCS
pRSET
Product Suggestions

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Videos
SDS
Documents

Documents

Product Information

Manuals

pRSET A, B, C Version: 18 June 2010 File size: 391 kB
Certificates
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Special Offers

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Invitrogen™ pRSET A, B, & C Bacterial Expression Vectors > 20μg each vector

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For Research Use Only. Not for use in diagnostic procedures.