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Invitrogen™ pIZT/V5-His Vector Kit

For high-level expression of the gene of interest

Brand:  Invitrogen™ V801001

Product Code. 10236542

  • 9550.00 NOK / 1 set

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Includes: The InsectSelect Glow Kit includes 20ÎĽg each of supercoiled, lyophilized pIZT/V5-His and pIZT/V5-His/CAT, 2ÎĽg each of lyophilized OpIE2 Forward and Reverse Sequencing primers, 1g Zeocin, Cellfectin Reagent, Sf9 cells, and 1 L of GIBCO Grace's Insect Media. The pIZT/V5-His Vector Kit contains only the expression vectors and primers as described above.
This item is not returnable. View return policy

Description

Description

  • The OpIE2 promoter for constitutive expression
  • The Zeocin resistance gene for rapid selection of stably transfected cell lines
  • C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Anti-V5 Antibody and rapid purification with nickel-chelating resin
  • In addition, pIZT/V5-His expresses a fusion of the green fluorescent protein and the Zeocin resistance protein (Zeo-GFP)
  • The Zeo-GFP fusion protein permits rapid selection of stably transfected cell lines with Zeocin and confers a fluorescent phenotype that simplifies identification of transfected cells

Constitutive Protein Expression in Insect Cells, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

TRUSTED_SUSTAINABILITY
Specifications

Specifications

OplE2
The InsectSelect™ Glow Kit includes 20 μg each of supercoiled, lyophilized pIZT/V5-His and pIZT/V5-His/CAT, 2 μg each of lyophilized OpIE2 Forward and Reverse Sequencing primers, 1 g Zeocin,™ Cellfectin™ Reagent, Sf9 cells, and 1 L of GIBCO™ Grace's Insect Media. The pIZT/V5-His Vector Kit contains only the expression vectors and primers as described above. Store frozen insect cells in liquid nitrogen. Store medium and Cellfectin™ Reagent at +4°C. Store all other reagents at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
His Tag (6x), V5 Epitope Tag
1 kit
InsectSelect™
Insect Cell Expression Vector
C-terminal
Restriction Enzyme/MCS
pIZ
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Manuals

InsectSelect Glow System with pIZT/V5-His Version: Version H 10/20/10 File size: 1,008.8 kB
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For Research Use Only. Not for use in diagnostic procedures.