missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Invitrogen™ pcDNA™3.1 Directional TOPO™ Expression Kit
Linearized, topoisomerase I-activated pcDNA3.1D/V5-His-TOPO vector for 5-minute directional cloning and subsequent high-level expression
Brand: Invitrogen™ K490001
6107.40 NOK valid until 2024-12-31
Use promo code "21651" to get your promotional price.
Includes: Box 1: 20μL pcDNA 3.1D/V5-His-TOPO (15–20ng/μL), 10μL dNTP Mix, 50μL Salt Solution, 1mL Sterile Water, 20μL T7 Sequencing Primer (0.1μg/μL), 20μL BGH Reverse Sequencing Primer (0.1μg/μL), 10μL Control PCR Template (0.1μg/μL), 10μL Control PCR Primers (0.1μg/μL each, 10μL Expression Plasmid (0.5μg/μL); Box 2: 6mL S.O.C. Medium, 21 × 50μL TOP10 Cell, 50μL pUC19 Control DNA
Description
- Proofreading enzyme is used: fewer errors in cloned genes
- Greater than 90% of clones are in correct orientation for expression, reducing time spent screening colonies for clone orientation
- CMV promoter: high expression levels
- C-terminal V5-His fusion tag for easy detection of recombinant protein with an Anti-V5 Antibody
- 6xHis tag for rapid protein purification on nickel-chelating resin
Constitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis
Order Info
Shipping Condition: Dry Ice
Specifications
| Constitutive | |
| CMV | |
| Geneticin™ (G-418) | |
| His Tag (6x), V5 Epitope Tag | |
| 20 Reactions | |
| TOPO™, pcDNA™ |
| Transfection | |
| TOPO Expression Kit | |
| Box 1 (Store at -20°C): • 20 μl pcDNA™3.1D/V5-His-TOPO™ (15–20 ng/μl) • 10 μl dNTP Mix • 50 μl Salt Solution • 1 ml Sterile Water • 20 μl T7 Sequencing Primer (0.1 μg/μl) • 20 μl BGH Reverse Sequencing Primer (0.1 μg/μl) • 10 μl Control PCR Template (0.1 μg/μl) • 10 μl Control PCR Primers (0.1 μg/μl each) • 10 μl Expression Plasmid (0.5 μg/μl) Box 2 (Store at -80°C): • 6 ml S.O.C. Medium (may be stored at 4°C or room temperature) • 21 × 50 μl TOP10 Cells • 50 μl pUC19 Control DNA |
|
| Directional TOPO | |
| Directional TOPO Vectors, pcDNA | |
| Constitutive Expression |
Product Content Correction
Your input is important to us. Please complete this form to provide feedback related to the content on this product.
Product Title
For Research Use Only. Not for use in diagnostic procedures.
Spot an opportunity for improvement?Share a Content Correction