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Invitrogen™ Human Cot-1 DNA™
Effective library-screening probe for somatic-cell hybrid libraries and flow-sorted chromosome libraries made from somatic-cell hybrids
Brand: Invitrogen™ 15279011
Includes: This DNA is supplied at 1mg/mL in 10mM Tris-HCl (pH 7.4), 1mM EDTA.
Description
Human Cot-1 DNA is commonly used to block nonspecific hybridization in microarray screening. It can also be used to suppress repetitive DNA sequences for the direct mapping of human DNA or mapping genomic clones to panels of somatic-cell hybrids for chromosome localization by Southern blotting.
About Cot-1 DNA
Human Cot-1 DNA is placental DNA that is predominantly 50 to 300 bp in size and enriched for repetitive DNA sequences such as the Alu and Kpn family members. The amount supplied per package is sufficient for 5–10 Southern or 500 in situ hybridizations.
Performance and quality testing
Purity and DNA size are verified by agarose gel electrophoresis. Concentration is verified spectrophotometrically by diluting Human Cot-1 DNA 1:100 in 50mM NaOH and using the conversion factor 0.033μg/μL/A260. Other methods used to determine concentration may yield varying results.
Array CGH, Cell Analysis, Cellular Imaging, ChIP-on-Chip, Chromatin Biology, Fluorescence In Situ Hybridization, Gene Expression Analysis and Genotyping, Genotyping and Genomic Profiling, In Situ Hybridization (ISH), Microarray Analysis, RNAi, Epigenetics and Non-Coding RNA Research
Order Info
Shipping Condition: Approved for shipment on Wet or Dry Ice
Specifications
| DNA QC | |
| Cot-1 DNA | |
| Human | |
| Block Non-Specific Hybridization |
| This DNA is supplied at 1 mg/ml in 10 mM Tris-HCl (pH 7.4), 1 mM EDTA. Store at -20°C. |
|
| Approved for shipment on Wet or Dry Ice | |
| Cot-1 DNA™ | |
| 500 μg |
For Research Use Only. Not for use in diagnostic procedures.