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Invitrogen™ pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ TOP10 E. coli

Delivers simple, 5-minute cloning and access to Gateway recombination cloning vector platform

Brand:  Invitrogen™ K250020

4486.89 NOK valid until 2025-03-21
Use promo code "24111" to get your promotional price.

Product Code. 10532893

  • 6730.00 NOK / 20 reactions

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To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”24111” to get your promotional price


Includes: TOPO-adapted, ready-to-use pCR8/GW/TOPO Vector, dNTPs, Salt Solution, Sterile Water, Gateway Forward and Reverse Sequencing Primers, Control Template and PCR Primers, TOP10 Chemically Competent E. coli Chemically Competent E. coli, S.O.C. Medium, and a pUC19 control plasmid.
This item is not returnable. View return policy

Description

Description

  • Contains minimal MCS, so sequence more of insert and less of vector
  • Patented att sites, pCR™8 vector to create entry clone, which facilitates easy access via Gateway recombination cloning to variety of expression vectors without tedious sub-cloning, saving valuable research time
  • att sites for rapid recombination into a variety of Gateway destination vectors
  • 3'-T overhangs for direct ligation of Taq-amplified PCR products
  • Sequencing primer sites within 55 bp of insertion site
  • Spectinomycin resistance gene for robust selection in E. coli
  • EcoRI sites flanking the PCR product insertion site for easy excision of inserts

Cloning,Gateway Cloning,PCR Cloning

TRUSTED_SUSTAINABILITY
Specifications

Specifications

TOPO™ with Gateway™
20 reactions
TA Cloning Kit
pCR
The pCR™8/GW/TOPO™ TA Cloning Kit contains the TOPO™-adapted, ready-to-use pCR™8/GW/TOPO™ Vector, dNTPs, Salt Solution, Sterile Water, Gateway Forward and Reverse Sequencing Primers, Control Template and PCR Primers, One Shot™ TOP10 Chemically Competent E. coli Chemically Competent E. coli, S.O.C. Medium, and a pUC19 control plasmid. Store the One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
One Shot™
20 Reactions
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Product Title
Invitrogen™ pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ TOP10 E. coli > 20 reactions

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For Research Use Only. Not for use in diagnostic procedures.