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Invitrogen™ pBAD-DEST49 Gateway™ Destination Vector
Designed for rapid cloning with Gateway entry clone using lambda phage site-specific recombination and subsequent high-level, tightly regulated expression in E. coli
Brand: Invitrogen™ 12283016
Includes: The pBAD-DEST49 Gateway destination vector includes 6μg of vector.
Description
- araBAD promoter for tightly regulated expression in E. coli
- HP-thioredoxin fusion for improved protein yield and solubility
- Enterokinase cleavage site for efficient cleavage of N-terminal fusion with EKMax™
- C-terminal V5 and 6xHis tags for efficient detection and purification of fusion proteins
- R sites for efficient recombination with any attL-flanked Gateway entry vector
Bacterial Expression/Cloning/Gateway Cloning/Protein Expression/Proteins, Expression, Isolation and Analysis
Specifications
| Inducible | |
| araBAD | |
| None | |
| Ampicillin (AmpR) | |
| His Tag (6x), V5 Epitope Tag, Thioredoxin | |
| 6 μg | |
| Gateway™ |
| Arabinose | |
| Gateway System Destination Expression Vector | |
| The pBAD-DEST49 Gateway™ destination vector includes 6 μg of vector. Store at -20°C. The vector is guaranteed stable for 6 months when properly stored. | |
| EK (Enterokinase) Recognition Site | |
| Gateway | |
| pBAD, pDEST |
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Product Title
Invitrogen™ pBAD-DEST49 Gateway™ Destination Vector > 6μg
For Research Use Only. Not for use in diagnostic procedures.
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