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Invitrogen™ FreeStyle™ 293-F Cells
Complete cell culture system for generating large amounts of mammalian recombinant protein
Brand: Invitrogen™ R79007
This item is not returnable.
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Description
Animal origin-free, chemically defined, low-protein, reduced growth factors, reduced serum, xeno-free. The FreeStyle 293-F cell line is derived from the 293 cell line and is intended for use with the FreeStyle MAX 293 Expression System or FreeStyle 293 Expression System. FreeStyle 293-F cells are adapted to suspension culture in FreeStyle 293 Expression Medium. Frozen cells are supplied in and may be thawed directly into FreeStyle 293 Expression Medium. The FreeStyle 293-F cell line is supplied in a vial containing 1mL of cells at 1 x 107 viable cells/mL in 90% FreeStyle 293 Expression Medium and 10% DMSO
Characteristics of FreeStyle 293-F cells:
- Prepared from low-passage Master Cell Bank cultures derived from parental 293-F cells that were recloned by limiting dilution
- The 293 clonal derived cultures are maintained in serum-free conditions for only 30 to 35 total passages
- Adapted to high-density, serum-free, suspension growth and may be maintained in FreeStyle 293 Expression Medium
- Demonstrate high transfection efficiencies with FreeStyle MAX reagent
- Suspension cultures may be transfected in FreeStyle 293 Expression Medium without the need to change media
- Permits transfection of cells at large volumes
- Voltage limit: 0.5-2.5kV
Antibody and Cell Culture Production, Bioproduction, Cell Culture, Mammalian Expression, Mammalian Expression Cell Lines, Mammalian Protein Production, Plasmid Transfection, Protein Expression, Proteins, Expression, Isolation and Analysis, Transfection
Specifications
| FreeStyle™ 293-F Cells | |
| 293-F | |
| Protein Expression and Transfection | |
| Freestyle™ | |
| 1 mL |
| 1 vial of 1 x 107 cells. Store in liquid nitrogen. | |
| Human | |
| FreeStyle™ 293 Expression Medium | |
| 293-F Cell | |
| FreeStyle™ |
For Research Use Only. Not for use in diagnostic procedures.