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Invitrogen™ Expressway™ Mini Cell-Free Expression System
Uses efficient coupled transcription and translation reaction to produce active recombinant protein
Brand: Invitrogen™ K990100
Includes: Designed to perform 20 x 50μL reactions or 1 x 1mL reaction. This system includes the IVPS E.coliExtract, IVPS reaction buffer, feed buffer, 19 amino acid mix, methionine, DNase/RNase-free water, RNase A, T7 Enzyme Mix, 2mL reaction tubes, and a positive expression control vector.
Description
- Eliminates time-consuming steps of cell-based protein production including transformation, cell culture, and expression optimization
- In two hours, can produce protein suitable for array of downstream applications and functional studies
- Offers flexibility in experimental design
- Synthesize proteins from both circular plasmid and linear templates (e.g. PCR products)
- Perform high-throughput synthesis of proteins
- Express genes toxic to in vivosystems
- Provides all components need for optimal cell-free protein production
- As DNA template is transcribed, 5« end of mRNA is bound by ribosomes and undergoes translation as 3« end of template is still being transcribed
- Proprietary feed buffer formulation is provided with system for increased protein yields
Cell Free Protein Expression for Functional Analysis, Cell-Free (in vitro) Expression, Protein Expression, Proteins, Expression, Isolation and Analysis
Specifications
| T7 | |
| Water Soluble Protein | |
| Cell-Free Expression | |
| Expressway™ |
| Cell-Free Expression System | |
| Cell-Free | |
| 1 kit |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Expressway Mini Cell-Free Expression System
Signal Word
- Warning
Hazard Category
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- Specific target organ toxicity Category 3
Hazard Statement
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H335-May cause respiratory irritation.
Precautionary Statement
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P312-Call a POISON CENTER/doctor/if you feel unwell.
- P337+P313-If eye irritation persists: Get medical advice/attention.
Supplemental information
- MIXTURE LIST-Contains : Tris (hydroxymethyl)aminomethane
For Research Use Only. Not for use in diagnostic procedures.