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Invitrogen™ Expressway™ Mini Cell-Free Expression System
Uses efficient coupled transcription and translation reaction to produce active recombinant protein
Brand: Invitrogen™ K990100
Includes: Designed to perform 20 x 50μL reactions or 1 x 1mL reaction. This system includes the IVPS E.coliExtract, IVPS reaction buffer, feed buffer, 19 amino acid mix, methionine, DNase/RNase-free water, RNase A, T7 Enzyme Mix, 2mL reaction tubes, and a positive expression control vector.
Description
- Eliminates time-consuming steps of cell-based protein production including transformation, cell culture, and expression optimization
- In two hours, can produce protein suitable for array of downstream applications and functional studies
- Offers flexibility in experimental design
- Synthesize proteins from both circular plasmid and linear templates (e.g. PCR products)
- Perform high-throughput synthesis of proteins
- Express genes toxic to in vivosystems
- Provides all components need for optimal cell-free protein production
- As DNA template is transcribed, 5« end of mRNA is bound by ribosomes and undergoes translation as 3« end of template is still being transcribed
- Proprietary feed buffer formulation is provided with system for increased protein yields
Cell Free Protein Expression for Functional Analysis, Cell-Free (in vitro) Expression, Protein Expression, Proteins, Expression, Isolation and Analysis
Specifications
T7 | |
Water Soluble Protein | |
Cell-Free Expression |
Cell-Free Expression System | |
Cell-Free | |
Expressway™ |
For Research Use Only. Not for use in diagnostic procedures.