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Invitrogen™ Dynabeads™ SILANE Viral NA Kit
For sensitive, highly predictable and consistent extraction and isolation of viral DNA and RNA from human serum/plasma samples, following a simple separation protocol.
Brand: Invitrogen™ 37011D
Includes: Dynabeads MyOne; SILANE (5mL, 40mg/mL, see Cat. no. 370-02D for further characteristics), binding/washing/elution buffers and a protocol optimized for isolation of viral DNA/RNA. The kit content is sufficient for 96 isolations.
Description
For sensitive, highly predictable and consistent extraction and isolation of viral DNA and RNA from human serum/plasma samples, following a simple separation protocol. The 1 μm Dynabeads have an optimized silica-like surface chemistry, and a high specific surface area, providing efficient kinetics and a high sensitivity. Their uniform size and surface area ensure reproducible results.
A higher sensitivity and improved performance is achieved with Dynabeads™ compared to magnetic particles from alternative suppliers. Other particles often have a random size range distribution, surface area and binding capacity. This could compromise the reproducibility of your results. Dynabeads™ hold reputable Dynal high standards with respect to within- and between-lot reproducibility and automation ability.
Advantages
• Isolates viral DNA and viral RNA
• High sensitivity, allowing for a low detection limit
• Effective isolation of nucleic acids even from challenging viruses (e.g. HBV)
• Can detect HBV DNA in a 200 μl serum sample containing only 40 infectious units (using 2 mg (50 μl) Dynabeads™)
• Quicker than spin-columns
• Cost-effective and performs to the same level as spin-columns
• Automation-friendly (slow sedimentation rate + high magnetic mobility)
Applications
• Isolation of viral nucleic acids (DNA/RNA)
• From human serum/plasma samples
• Well suited for automated assays
• Separate kit available for the isolation of genomic DNA (Cat. no. 370-12D)
Additional Info
Validation and customization (bead, buffer, protocol or format) can be made available on a custom OEM basis. For further information, please contact us at: ivd@invitrogen.com
Order Info
Shipping Condition: Room Temperature
Specifications
| • 5 mL Dynabeads MyOne SILANE; 4°C • 30 mL Lysis/binding Buffer; 4°C • 2 x 45 mL Washing Buffer 1; 4°C • 30 mL Washing Buffer 2; 4°C • 10 mL Elution Buffer; 4°C , The kit contains: Dynabeads™ MyOne™ SILANE (5 mL, 40 mg/mL, see Cat. No. 370-02D for further characteristics), binding/washing/elution buffers and a protocol optimized for isolation of viral DNA/RNA. The kit content is sufficient for 96 isolations. |
|
| Plasma, Serum | |
| Viral DNA and RNA | |
| RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Southern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, hybridization | |
| 96 preps | |
| 1 μm | |
| Up to 45 min. |
| Magnetic Bead | |
| 100 μL | |
| Automated Liquid Handling Systems | |
| High-throughput Compatible | |
| Room Temperature | |
| Viral DNA/RNA | |
| Serum: 200 μL Plasma: 200 μL |
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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