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Invitrogen™ Colorimetric ReadiLAMP™ Kit, SARS-CoV-2
The Invitrogen Colorimetric ReadiLAMP Kit for SARS-CoV-2 is a loop-mediated isothermal amplification (LAMP)-based solution for rapid SARS-CoV-2 detection in saliva and nasopharyngeal/nasal swab samples.
Brand: Invitrogen™ A52539
This item is not returnable.
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Description
- The Invitrogen Colorimetric ReadiLAMP Kit for SARS-CoV-2 is a loop-mediated isothermal amplification (LAMP)-based solution for rapid SARS-CoV-2 detection in saliva and nasopharyngeal/nasal swab samples
- The first protocol option provides a workflow for RNA purification followed by a colorimetric LAMP assay
- For robust SARS-CoV-2 RNA isolation, the MagMAX Viral/Pathogen II Nucleic Acid Isolation Kit (Cat. No. A48383) or PureLink Viral RNA/DNA Mini Kit (Cat. No. 12280050) are recommended
- The second protocol option consists of a simple SARS-CoV-2 viral lysis method for compatible sample types followed by a colorimetric LAMP assay
- This protocol does not require nucleic acid isolation or complex sample preparation; instead it directly incorporates the saliva or nasopharyngeal/nasal swab samples as LAMP assay input
Features of the Colorimetric ReadiLAMP Kit for SARS-CoV-2 include:
- Simplicity—Detection is fast, simple, and intuitive; LAMP reaction changes color from purple to blue if SARS target is present
- No instrumentation required, except for a 65 heating block
- Sensitivity—Input of up to 50% volume of purified RNA per LAMP reaction, enabling maximal sensitivity in SARS-CoV-2 detection
- Direct LAMP: ≤250 copies per reaction (25 μL)
- Purified RNA-based LAMP: ≤100 copies per reaction (25 μL)
- Speed—Both protocols can be completed within 1 hour
- Direct LAMP: ∼30 minutes using crude saliva or swabs in non-serum-containing transport media
- Purified RNA-based LAMP: ∼60 minutes (∼30 minutes RNA purification, followed by 30 minutes LAMP assay)
- Throughput—Process between 1 and 96 samples at a time, enabling the processing of more samples with same personnel headcount, using individual tubes, tube strips, or 96-wells
Specifications
Kit does not contain beads. Recommending the use of RNA extraction MVP II kit which contains magnetic beads | |
During reaction color changes from purple to blue | |
Saliva or Nasopharyngeal/Nasal Swab | |
SARS-CoV-2 surveilance | |
Heating block | |
Detects SARS-CoV-2 presence | |
SARS-CoV-2 | |
100 |
<250 copies/25 μL (Direct LAMP) <100 copies/25 μL (Purified RNA-based LAMP) |
|
Colorimetric readout | |
SuperScript IV & proprietary Bst-derived DNA polymerase | |
Liquid. 5 components included in the kit. | |
LAMP Buffer (5x), Enzyme Mix (10x), SARS-CoV-2 primer mix (5x), Human Control primer mix (5x), SARS-CoV-2 control RNA | |
Range of sample inputs | |
For SARS-CoV-2 detection. |