Perform hapten-carrier conjugation with cysteine-containing peptides and other thiolated antigens with this sulfhydryl-reactive mariculture KLH.
Brand: Thermo Scientific™ 77666
Thermo Scientific™ Imject Maleimide-Activated mcKLH is sulfhydryl-reactive immunogenic mariculture keyhole limpet hemocyanin (KLH) for hapten-carrier conjugation with cysteine-containing peptides and other thiolated antigens.
The high-quality mariculture KLH is rendered sulfhydryl-reactive by chemical modification with Sulfo-SMCC crosslinker, and the resulting maleimide-activated protein is then stabilized and lyophilized in phosphate buffer. Maleimide groups form covalent crosslinks with sulfhydryl (-SH) moieties on cysteine residues of peptides and other thiol-containing haptens. Numerous antigen molecules can be conjugated to each KLH molecule to make them highly immunogenic and increase the likelihood of eliciting immunized animals to produce high-titer antisera to the epitopes of interest. Select from among several package sizes of activated KLH protein, or choose the complete conjugation kit that includes optimized buffers and trouble-free spin desalting columns.Highlights:
Convenient packages – choose stabilized, lyophilized Maleimide-Activated mcKLH or a complete immunogen conjugation kit
Optimized activation – specifications require that each lot is activated with more than 400 moles of sulhydryl-reactive maleimide per mole of KLH, ensuring consistently high levels of conjugation
Sulfhydryl-reactive – engineered for conjugating cysteine-containing peptide antigens to make effective immunogens for high-titer antibody production
Simple, rapid procedure – complete the hapten-carrier conjugation reaction in two hours to yield sufficient immunogen for several injections and booster immunizations of host animals
High-quality carrier protein – KLH is isolated from the mollusk Megathura crenulata (keyhole limpet); mcKLH is a high-quality form of KLH that is harvested from limpets grown in mariculture
Highly immunogenic – KLH has a high molecular mass (0.5 to 13 million daltons; aggregates of 350 and 390kDa subunits) and elicits a stronger immune response than BSA or ovalbumin
Excellent solubility – our preparations of mariculture KLH are more soluble than typical forms and we also offer a PEGylated form for even greater hydrophilicity and solubility
Includes:
Kit contains stabilized, lyophilized Maleimide-Activated mcKLH (in PBS with stabilizer), buffers and desalting columns to easily prepare ready-to-use immunogens for immunization
| Sufficient For: 5 conjugation reactions and complete immunogen preparations, each with up to 2 mg sulfhydryl-containing peptide • Imject Maleimide-Activated mcKLH, 5 x 2 mg • Imject Maleimide Conjugation Buffer, 30 mL • Imject Purification Buffer Salts, 5 x 0.84 g • Zeba Spin Desalting Columns, 7K MWCO, 2 mL, 5 columns Store the Maleimide-Activated blue Carrier Protein at -20°C. Store remaining components at 4°C. |
|
| Kit | |
| Imject™ |
| Imject Maleimide Activated mcKLH Spin Kit | |
| 5 x 2 mg |
Catalog No. 10399053
Catalog No. 10678484
Catalog No. 10671531
Catalog No. 13464189
Catalog No. 10647894
Catalog No. 10116287
Catalog No. 17821163
Gibco ™ Human FGF-basic (FGF-2/bFGF) (154 aa) Recombinant Protein, PeproTech Gibco ™ Human FGF-basic (FGF-2/bFGF) (154 aa) Recombinant Protein, PeproTech
Catalog No. 11570806
Catalog No. 17833373
Gibco ™ Human EGF, Animal-Free Recombinant Protein, PeproTech Gibco ™ Human EGF, Animal-Free Recombinant Protein, PeproTech
Catalog No. 11804081
Catalog No. 11800094
Catalog No. 10475685
A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.
Your input is important to us. Please complete this form to provide feedback related to the content on this product.
For Research Use Only. Not for use in diagnostic procedures.
