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Cytiva Hot Start Master Mix
A ready-to-use 2X mastermix providing robust and reliable performance for demanding PCR applications in which high specificity and high sensitivity are essential to success
Brand: Cytiva 25-1500-01
This item is not returnable.
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Description
- Convenient, ready-to-use mix saves time and reduces experimental variability
- For any standard PCR reaction, such as multiplex PCR, and reactions exhibiting nonspecific amplification or primer-dimer formation
- Primer sequestration effectively reduces any nonspecific priming or primer-dimer formation
- Recombinant activator protein avoids the risk of contamination from a mammalian source associated with hot start antibodies
- Avoids the risk of contamination from a mammalian source associated with hot start antibodies
- Quick hot start activation preserves sample integrity by avoiding extensive precycling heat treatment
Specifications
| 2X Concentrate solution of 20mM Tris HCl (pH 8.6), 100mM KCl, 3mM MgCl2, 0.4mM dNTPs (dATP, dCTP, dGTP, dTTP), 50U/mL Taq DNA Polymerase, hot start activator protein, stabilizers. Store at -20°C | |
| Solution | |
| 100 Reactions | |
| 100 |
| Hot Start Master Mix is a 2X premixed formulation that can effectively reduce nonspecific priming and primer-dimer formation during PCR. | |
| Solution | |
| Standard PCR | |
| 50μL |