Gibco™ Human MRP8 Vesicles

Description

Human MRP8 Vesicles are insect derived purified plasma membranes with inserted MRP8 transport protein (Multidrug Resistance-associated Protein 8, ABCC11). Our Human MRP8 Vesicles can be used to evaluate test compounds and drug interactions with MRP8 in in vitro assays. MRP8 is an ATP-binding cassette (ABC) transporter that carries organic anions.

Use Human MRP8 Vesicles to:

• Investigate the transporter interactions of your drug candidates
• Assess potential for transporter-mediated drug-drug interactions
• Obtain high quality results with a large signal to noise ratios

Concept Behind ABC Transporter Vesicles

ABC transporter vesicles are easy-to-use, efficient reagents for early assessment of a drug candidate's substrate and drug interaction potential. While ABC transporters typically mediate the export of substrates out of cells, transporters expressed on these inside-out vesicles import substrates into the vesicles. It is therefore possible to quantitatively evaluate transport activity for your compound by determining the amount incorporated into the vesicles.

Clear and Reliable Results

Prepared from Sf9 cells which have been engineered to over-express specific ABC transporters, these 'inside-out' vesicles provide high levels of transporter activity with low background, giving you a clear signal if your compound is a substrate or inhibitor of a specific efflux transporter.

Transporter Vesicle Details

Human MRP8 Vesicles are part of a larger group of transporter vesicles known as ABC transporter vesicles. They are prepared from purified plasma membranes isolated from an insect cell system (Sf9 cells transfected with baculovirus) expressing MRP8. MRP8 is highly expressed in the liver, where it may be associated with drug excretion. It has been reported that MRP8 expression is increased in lung cancer, and the MRP8 gene may be involved in multidrug resistance to anti-cancer drugs.