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Invitrogen™ Human EGF ELISA Kits
Measure levels of epidermal growth factor (EGF) in human serum, plasma, and cell culture supernatant using 96-well plates.
Brand: Invitrogen™ EHEGF
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Description
Assay EGF (epidermal growth factor) levels in human serum, plasma (EDTA, heparin, sodium citrate), and culture supernatant samples with our Invitrgen™ Human EGF ELISA Kits.
These human EGF ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of epidermal growth factor (EGF, also called URG, HOMG4) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. The antibody-pair kit consists of the matched EGF antibody pairs and EGF standard only. All components undergo quality control testing to minimize lot-to-lot variability.Target: Natural and recombinant human epidermal growth factor (EGF); also known as urogastrone (URG), hypomagnesemia type 4 (HOMG4)
Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: Absorbance at 450nm minus absorbance at 550nm
Assay Range: 7.8 to 500pg/mL
Sensitivity: ≤ 5pg/mL
Reproducibility: Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
Sample Types: Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)
Total Assay Time: 3.5 hours
Sample Size: 50μL
Specificity: No significant cross-reaction with human HB-EGF (Heparin-binding EGF), mouse EGF, rat EGF and human IL-2, IL-4, IL-6, IL-8, TNFα TGFβ, GM-CSF
Includes:Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a “Screening Set”) contains matched antibodies, standard and detection reagents but no coated plates. The Antibody Pair Kit (previously called a “MiniKit”) contains only matched antibodies and standard (no plates or detection reagents).
Specifications
| 7.8 to 500pg/mL | |
| Human EGF ELISA Kit | |
| Cell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum, Urine | |
| 96 microplate wells | |
| Absorbance at 450nm minus absorbance at 550nm | |
| Natural and recombinant human epidermal growth factor (EGF); also known as urogastrone (URG), hypomagnesemia type 4 (HOMG4) |
| ≤ 5pg/mL | |
| Colorimetric ELISA (TMB substrate) with Plates | |
| No significant cross-reaction with human HB-EGF (Heparin-binding EGF), mouse EGF, rat EGF and human IL-2, IL-4, IL-6, IL-8, TNFa TGFß, GM-CSF | |
| Stop Solution, 13mL, 30X Wash Buffer, 50mL, TMB Substrate Solution, 13mL, Streptavidin-HRP Concentrate, 75µL, Standard Diluent, 14mL, Lyophilized Recombinant Human EGF Standard, 2 vials, HRP Dilution Buffer, 14mL, Anti-Human EGF Precoated 96-well Strip Plate, 1 ea, Adhesive Plate Covers, 6 ea, Biotinylated Antibody Reagent, 8mL | |
| 1 Plate Kit |