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Thermo Scientific™ HisPur™ Ni-NTA Superflow Agarose
Perform FPLC purification of poly-histidine-tagged proteins with this nitrilotriacetic acid (NTA) modified Superflow 6 support charged with divalent nickel (Ni+2).
Brand: Thermo Scientific™ 25215
3483.10 NOK valid until 2024-11-30
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Description
Perform FPLC purification of poly-histidine-tagged proteins with Thermo Scientific™ HisPur Ni-NTA Superflow Agarose, a nitrilotriacetic acid (NTA) modified Superflow 6 support charged with divalent nickel (Ni+2).
Additional Information and Online Ordering
HisPur Ni-NTA Superflow Agarose is a highly crosslinked, durable resin that does not compress at flow rates used for medium- or large-scale FPLC purifications. The resin holds up well to a variety of chemicals and pH values, and is compatible with common clean-in-place procedures. Compared to cobalt and other ligands used for immobilized metal affinity chromatography (IMAC), nickel provides greater capacity for His-tagged protein purification. HisPur Ni-NTA Superflow Agarose exhibits a high dynamic binding capacity across a range of flow rates, making it an excellent choice for larger scale purifications.Highlights:
High capacity – binds greater than 20mg of His-tagged GFP per mL of resin at a linear flow rate of 300cm/hour
High purity – provides greater than 80% purity of eluted fractions when used to purify from lysates
Versatile – can be used to purify proteins under native and denaturing conditions
Robust – highly crosslinked beads tolerate linear flow rates up to 1200cm/hour
Compatible – maintains function in a wide variety of chemicals and pH conditions
Cost effective – competitively priced resin is stable through multiple cycles of cleaning and reuse
Recommended for:
Medium- to large-scale FPLC purification of polyhistidine tagged proteins
Specifications
| 20 mg His-Tagged GFP Binding per mL Resin | |
| Liquid Suspension | |
| 50 mL | |
| Agarose | |
| Bottle |
| Store at 4°C | |
| HisPur™ | |
| His-Tagged Protein | |
| Divalent Nickel, Metal Chelate | |
| Affinity, Agarose Resin |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- HisPur Ni-NTA Superflow Agarose, 50 mL.
Signal Word
- Danger
Hazard Category
- Flammable liquid Category 3
- Skin sensitiser Category 1
- Respiratory sensitiser Category 1
- Carcinogenicity Category 1
- Reproductive toxicity Category 1B
- Specific target organ toxicity after repeated exposure Category 2
- LONG-TERM AQUATIC HAZARD Chronic 3
Hazard Statement
- H226-Flammable liquid and vapour.
- H317-May cause an allergic skin reaction.
- H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
- H350-May cause cancer.
- H360D-May damage the unborn child.
- H373-May cause damage to organs.
- H412-Harmful to aquatic life with long lasting effects.
Precautionary Statement
- P201-Obtain special instructions before use.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P302+P352-IF ON SKIN: Wash with plenty of water/.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P370+P378-In case of fire: Use to extinguish.
Supplemental information
- MIXTURE LIST-Contains : Ethyl alcohol, Nickel(II) sulfate hexahydrate(1:1:6)
For Research Use Only. Not for use in diagnostic procedures.