Thermo Scientific™ HisPur™ Cobalt Superflow Agarose

Description

Perform high-purity, large-scale purification of polyhistidine-tagged proteins with Thermo Scientific™ HisPur Cobalt Superflow Agarose, a tetradentate-chelating Superflow 6 support charged with divalent cobalt.

Additional Information and Online Ordering

Highlights:

• Robust – highly crosslinked 6% agarose Superflow beaded support tolerates linear flow rates up to 1200cm/hour with minimal leaching of cobalt due to a tetradentate chelator

• High yields – binds 20mg of 6xHis-GFP per mL of resin at a linear flow rate of 150cm/hour, can bind greater than 30mg per mL of resin depending on flow rate

• High purity – specificity of cobalt binding to histidine-tag generally results in elution fractions with less than 10% contamination of non-specific proteins

• Compatible – maintains function after exposure to a wide variety of chemicals and pH values

• Cost effective – resin is stable through multiple cycles of cleaning and reuse

Recommended for:

Large scale FPLC purification of polyhistidine tagged proteins