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  6. Cytiva HiPrep™ Sephacryl™ HR Prepacked Gel Filtration Columns

Cytiva HiPrep™ Sephacryl™ HR Prepacked Gel Filtration Columns

For reproducible protein purification, separating polysaccharides, macromolecules with extended structures and small plasmids

8875.00 NOK - 12685.00 NOK

Specifications

Quantity Each
Particle Size 25 to 75 μm
For Use With (Equipment) Low to medium pressure AKTA™ systems or pumped chromatography systems
Product Type Sephacryl HR Pre-packed Gel Filtration Column
For Use With (Application) Reproducible Protein purification, separating Polysaccharides, macromolecules with extended structures and small plasmids
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Products 3
Product Code Brand Flow Rate Bed Volume Diameter (Metric) Inner Price Quantity & Availability  
Product Code Brand Flow Rate Bed Volume Diameter (Metric) Inner Price Quantity & Availability  
11350292
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Cytiva
28-9356-06 		<1.3 mL/min. 120 mL 1.6cm
8875.00 NOK
Each
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11330292
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Cytiva
28-9356-04 		<0.5 mL/min. 120 mL 1.6cm
8875.00 NOK
Each
In Stock
to see stock.
Add to basket
 
11360292
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Cytiva
28-9356-07 		<0.5 mL/min. 320 mL 2.6cm
12685.00 NOK
Each
In Stock
to see stock.
Add to basket
 
Description

Description

Aids in preparative separation of biomolecules from up to 5mL (for 16mm column I.D.) and 13mL (for 26mm column I.D.).

  • Provides maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability
  • Prepacked columns for use with AKTAdesign™ or other pumped chromatography systems
  • Available in two different prepacked column sizes - 16 x 600mm (120mL) and 26 x 60mm (320mL)
  • Maximum pressure (over packed bed during operation) is 1.5 bar (22psi)

Media    

  • Average particle size: 50μm
  • Matrix: Allyl dextran and N, N'-methylenebisacrylamide
  • pH Stability working range: 3 to 11
  • pH Stability cleaning range: 2 to 13
  • Chemical stability: all commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1% SDS, 20% ethanol, 30% propanol, 30% acetonitrile (tested at 40°C for one week), 0.5 M NaOH (only for cleaning-in-place)
  • Pressure/Flow specification: >125cm/hr., 100kPa, XK 50/30, bed height 15cm
Column
  • Material (bed support): Polymethyl-Methacrylate

HiPrep 16/60 Sephacryl S-400 HR

  • Media:
  • Fractionation [Mr] globular proteins: 2 x 104-8 x 106
  • Exclusion limit [Mr] (globular proteins): ≈9 x 106 (9000kDa)
  • Fractionation range [Mr] DNA exclusion limit:    271bp
  • Fractionation [Mr] Dextrans: 1 x 104-2 x 106
HiPrep 16/60 Sephacryl S-500 HR
  • Media:
  • Exclusion limit for Dextrans: 100 x 106
  • Fractionation range [Mr] DNA exclusion limit: 1078bp
  • Fractionation [Mr] Dextrans: 4 x 104-2 x 107

Specifications

Specifications

Each
Low to medium pressure AKTA™ systems or pumped chromatography systems
Reproducible Protein purification, separating Polysaccharides, macromolecules with extended structures and small plasmids
60cm
1.5 bar [0.15 MPa] (22 psi)
Allyl Dextran and N,N'-methylenebisacrylamide
25 to 75 μm
Sephacryl HR Pre-packed Gel Filtration Column
23.62 in.
Polypropylene
3 to 11
Polysaccharides, macromolecules with extended structures and even small plasmids
Videos
Safety and Handling

Safety and Handling

Product Identifier
  • HiPrep 16/60 Sephacryl S-500 HR

Signal Word
  • Warning

Hazard Category
  • Flammable liquid Category 3

Hazard Statement
  • H226-Flammable liquid and vapour.

Precautionary Statement
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P235-Keep cool.
  • P501-Dispose of contents/container to .

Supplemental information
  • MIXTURE LIST-Contains : ethanol

SDS
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