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Invitrogen™ HCK Human Protein
Easily screen for kinase binding. Thermo Fisher Invitrogen™ HCK Human Protein fusion protein consists of the catalytic domain of transforming growth factor beta receptor 2 (TGFBR2) and glutathione S-transferase (GST) tag. It is validated in the LanthaScreen™ Eu Kinase Binding Assay.
5160.00 NOK - 69300.00 NOK
Specifications
| Accession Number | NP_002101 |
|---|---|
| Formulation | 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.5mM EDTA, 0.02% NP-40, 2mM DTT and 50% glycerol. |
| Molecular Weight (g/mol) | 84.8kDa |
| Storage Requirements | Store at -80°C. At first use, aliquot and store at -80°C to avoid multiple freeze-thaws. |
| Structural Form | Full Length |
| Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
|---|---|---|---|---|---|---|---|---|---|
| Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
|
11330872
|
Invitrogen™
PV6128 |
10 μg |
5160.00 NOK
Each |
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|
15418723
|
Invitrogen™
PR9171A |
100 μg |
16000.00 NOK
100µg |
Please sign in to purchase this item. Need a web account? Register with us today! | |||||
|
15438813
|
Invitrogen™
PV6129 |
1 mg |
69300.00 NOK
1mg |
Please sign in to purchase this item. Need a web account? Register with us today! | |||||
Description
The LanthaScreen™ Eu Kinase Binding Assay is a TR-FRET assay that lets you measure inhibitor binding to non-activated kinases, perform simple off-rate measurements and makes simple the study of type III (allosteric) inhibitors.
The LanthatScreen™ Eu Kinase Binding Assay is easy to optimize and run. The assay uses a simple mix-and-read format with a single one-hour incubation and no development steps. Simultaneous binding of an Alexa Fluor™ conjugate or tracer” to a tagged kinase is detected by addition of a europium-labeled anti-tag antibody, in this case anti-GST. Simultaneous binding of the tracer and antibody to the kinase results in a high degree of fluorescence resonance energy transfer (FRET), while displacement of the tracer with a kinase inhibitor results in a loss of FRET.
- Compatible and validated with the LanthaScreen™ Eu Kinase Binding Assay
- Expressed in insect cells
- GST tagged for use with Eu-labeled GST antibody
- No special measures were taken to activate this kinase
TGFBR2 for the LanthaScreen Kinase Binding Assay
Transforming Growth Factor, beta receptor 2 (TGFBR2) is a member of the TGF-beta family of receptor Serine/Threonine kinases. It is involved in the regulation of cell proliferation. (TGFBR2 Aliases: AAT3, FAA3, MFS2, RIIC, LDS1B, LDS2B, TAAD2, TGFR-2, TGFbeta-RII).
This insect cell-expressed recombinant fusion protein of the TGFBR2 catalytic domain and GST tag has been validated in the LanthaScreen™ Eu Kinase Binding Assay with the Tracer 236 and Eu-labeled GST Antibody reagents.
Specifications
| NP_002101 | |
| 84.8kDa | |
| Full Length | |
| SRC Kinase Family | |
| Recombinant | |
| Baculovirus |
| 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.5mM EDTA, 0.02% NP-40, 2mM DTT and 50% glycerol. | |
| Store at -80°C. At first use, aliquot and store at -80°C to avoid multiple freeze-thaws. | |
| HCK | |
| Human | |
| GST Tag | |
| Frozen |