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Thermo Scientific™ Halt™ Phosphatase Inhibitor Single-Use Cocktail
Stabilize serine, threonine and tyrosine phosphorylation of proteins in cells and tissues with this cocktail of phosphatase inhibitors.
Brand: Thermo Scientific™ 78426
3713.30 NOK valid until 2024-12-20
Use promo code "21651" to get your promotional price.
This item is not returnable.
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Description
Preserve phosphorylation of proteins in cells and tissues with Thermo Scientific™ Halt Phosphatase Inhibitor Cocktail.
- Halt Phosphatase Inhibitor Cocktail protects phosphoproteins from serine/threonine phosphatases, as well as protein tyrosine phosphatases (PTPs) during cell lysis
- The cocktail contains a mixture of four inhibitors of broad specificity including sodium fluoride, sodium orthovanadate, sodium pyrophosphate, and beta-glycerophosphate
- Conveniently packaged in a 100X format, the cocktail is stable at 4°C and is suitable for use with any cell or tissue extract
- The cocktail preserves protein phosphorylation in immunoprecipitation and kinase assays
- Phosphorylation and dephosphorylation comprise a molecular on/off switch that regulates many key biological pathways within the cell including signal transduction, cell division, and apoptosis
- To effectively study phosphorylation activation states, one must inactivate phosphatases to prevent their uncontrolled activity upon cell lysis
Additional Features
- Devoid of protease inhibitors – To protect against both proteases and phosphatases, use Halt Protease and Phosphatase Inhibitor Cocktail (78420)
- Dialyzable – Either dialyze or desalt sample to effectively remove inhibitors from cell extracts before performing procedures that are discovered to be incompatible with cocktail components
- Supplied as a 100X concentrate – effective when used at a final concentration of 1X; however, samples that contain particularly high levels of phosphatase activity may require a final concentration of 2-3X
- Compatibility – compatible with most detergent-based cell lysis reagents, including all Thermo Scientific Pierce Cell Lysis Solutions
- All-in-one format – inhibits both serine-threonine phosphatases and protein tyrosine phosphatases
- Compatible with popular protein assays – works with standard protein assays, including BCA and coomassie (Bradford) assays
- Convenient storage – refrigerator storage (2 to 8°C) saves valuable space in the freezer and allows for immediate use
Specifications
Phosphatase Inhibitor Cocktail | |
Liquid | |
Phosphatase Inhibitor | |
5 x 1 mL |
Upon receipt store product at 4°C. Do not freeze. | |
Halt™ | |
Phosphatase Inhibitor |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Halt Phosphatase Inhibitor Cocktail
Signal Word
- Warning
Hazard Category
- Acute toxicity Category 4
Hazard Statement
- H302-Harmful if swallowed.
Precautionary Statement
- P264-Wash hands thoroughly after handling.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : sodium fluoride, trisodium tetraoxovanadate
For Research Use Only. Not for use in diagnostic procedures.