Immobilize polyclonal antibodies or other glycoproteins, via periodate-oxidized sugars, onto hydrazide-activated polyacrylamide beads for AP, IP or pull-down assays.
Brand: Thermo Scientific™ 88941
Thermo Scientific™ GlycoLink Immobilization Kits provide affinity columns and reagents to immobilize glycoproteins through oxidized sugar groups for use in affinity-purification applications or studies of protein-protein interactions.
The GlycoLink Kits use hydrazide-activated resin and a special catalyst to prepare affinity columns with polyclonal antibodies, which typically have abundant carbohydrates (glycosylation) on their Fc portions. Any glycosylated proteins (even monoclonal antibodies) that contain periodate-oxidizable carbohydrate groups can be immobilized with these kits to make reusable affinity columns. Antibodies immobilized by this hydrazide immobilization chemistry have unobstructed antigen-binding sites and optimal purification capability. Prepared columns with stable glycoproteins or antibodies can be regenerated and reused at least five times for affinity-purification without significant loss in binding capacity.Highlights:
High-capacity – immobilize 1 to 10mg of oxidized antibody or other glycoprotein per milliliter of resin
Fast and efficient – immobilize in as few as 30 minutes; achieve at least 90% coupling of most glycoproteins in less than 4 hours (efficiency depends on the amount and type of glycosylation)
Specific immobilization – the hydrazide-activated UltraLink Resin conjugates only to purified glycoproteins whose sugar (e.g., sialic acid) groups have been gently oxidized with periodate
Stable attachment – resonance structure of the hydrazone bonds are sufficiently stable for multiple affinity purifications with one batch of prepared resin; no stabilization step is required
Preserves antibody function – immobilizes IgG via the Fc region, thereby keeping both antigen binding sites available for capturing target
Convenient sizes – choose from two complete 10-column kits: one with 1mL columns and one with 0.1mL columns
UltraLink Hydrazide Resin:
Includes:
Kits contain GlycoLink Columns; coupling buffer, coupling catalyst and wash buffer; oxidizing reagent; desalting columns, PBS buffer pack; and column accessories
Recommended for:
Purify antigens using covalently coupled antibodies; Determine binding partners for a glycoprotein of interest; Perform large-scale affinity purifications
| 1 to 10 mg of GlycoProtein Immobilization per Column | |
| Sufficient For: Immobilizing 1 to 10 mg of glycoprotein per column • GlycoLink Columns, 1 mL, 10 columns • GlycoLink Coupling Buffer, 2 x 100 mL • Aniline, 0.2 mL • Wash Buffer, 120 mL • Sodium meta-periodate, 0.5 g • Zeba Spin Desalting Columns, 5 mL, 10 columns • PBS Pack (makes 500mL), 1 pack • Column Accessories, 10 ea Store at 4°C. |
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| 10 columns kit | |
| Hydrazide | |
| Spin Column |
| GlycoLink Immobilization Kit | |
| GlycoLink™ | |
| Immobilization Kit | |
| 10 Columns | |
| Affinity, UltraLink™ Resin |
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For Research Use Only. Not for use in diagnostic procedures.
