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Gibco™ RPMI 1640 Medium, GlutaMAX™ Supplement

Description
Requires
Supplementation
RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium) was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This RPMI 1640 is modified as follows:
With: GlutaMAX™, Phenol Red
Without: HEPES
The complete formulation is available.
Using RPMI 1640 Medium
RPMI 1640 medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 medium with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. RPMI 1640 medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 medium uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Order Info
Shipping Condition: Room Temperature
- Environmental benefits include:
- Source Reduction
- Recyclable

Specifications
Specifications
Cell Line | HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas |
Cell Type | Leukemic Cells |
Classification | Animal Origin-free |
Concentration | 1 X |
Form | Liquid |
Product Type | RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium) |
Serum Level | Standard Serum Supplementation |
Sterility | Sterile-filtered |
Sterilization Method | Sterile-filtered |
With Additives | GlutaMAX, Phenol Red |
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Certificates
Certificates
A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.
Lot Number | Certificate Type | Date | Product Code |
---|---|---|---|
2541951 | Certificate of Analysis | 31/01/2025 | 61870036,61870143,61870150 |
2518039 | Certificate of Analysis | 29/01/2025 | 61870036 |
2522769 | Certificate of Analysis | 29/01/2025 | 61870036 |
2652534 | Certificate of Analysis | 29/01/2025 | 61870036 |
2436538 | Certificate of Analysis | 29/01/2025 | 61870036,61870143,61870150 |
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For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
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