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Gibco™ Recovery™ Cell Culture Freezing Medium
Complete, ready-to-use freezing medium for cryopreservation of a wide variety of mammalian cells, including CHO-S, CHO-K1, HEK 293, Jurkat, and NIH 3T3
Brand: Gibco™ 12648010
936.04 NOK valid until 2025-06-27
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Description
For many years, researchers have used a common recipe for making freezing medium: High-glucose Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% serum and 10% DMSO. Recovery Cell Culture Freezing Medium is an optimized version of this classic formulation, using Gibco Bovine Serum and Fetal Bovine Serum to provide improved cell recovery and viability after thawing.
- Suitable for a wide variety of mammalian cells
- Can be used to freeze most mammalian cells cultured in DMEM, DMEM/F-12, MEM, RPMI 1640, and Ham's F-12
- Quality tested for pH, osmolality, sterility, and endotoxin
- Each lot is performance tested using CHO-K1 cells
Cell Counting, Viability & Cryopreservation, Cell Culture, Mammalian Cell Culture, Gene Expression Analysis & Genotyping, In Vitro Transcription, PCR & Real-Time PCR
Order Info
Shipped on dry ice.
Compliance
Manufactured at a cGMP-compliant facility registered with the FDA as a medical device manufacturer and certified to ISO 13485 standards.

Specifications
Mammalian Cells | |
Freezing Medium | |
Sterile-filtered | |
No Phenol Red | |
50 mL | |
Research Grade | |
Mammalian Cell Culture |
Liquid | |
Standard Serum Supplementation | |
High Glucose, Phenol Red, DMSO (10%) | |
Gibco™, Recovery™ | |
Dry Ice | |
Storage conditions: -5 to -20°C. Protect from light. Shipping conditions: Frozen Shelf life: 12 months from date of manufacture |
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Safety and Handling
- 3-Methyl-1-butanol
Signal Word
- Warning
Hazard Category
- Acute toxicity Category 4
- Serious eye damage/eye irritation Category 2
- Flammable liquid Category 3
- Skin corrosion/irritation Category 2
- Specific target organ toxicity Category 3
Hazard Statement
- H226-Flammable liquid and vapour.
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H332-Harmful if inhaled.
- H335-May cause respiratory irritation.
- EUH066-Repeated exposure may cause skin dryness or cracking.
Precautionary Statement
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P337+P313-If eye irritation persists: Get medical advice/attention.
Supplemental information
- MIXTURE LIST-Contains : 3-Methyl-1-butanol
Certificates
A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.
Lot Number | Certificate Type | Date | Catalog Number |
---|---|---|---|
2596542 | Certificate of Analysis | 31/01/2025 | 12648010 |
2584605 | Certificate of Analysis | 29/01/2025 | 12648010 |
2584603 | Certificate of Analysis | 29/01/2025 | 12648010 |
2584609 | Certificate of Analysis | 29/01/2025 | 12648010 |
2584607 | Certificate of Analysis | 29/01/2025 | 12648010 |
Promotions
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For Research Use Only. Not for use in diagnostic procedures.