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Description
MEM (Minimum Essential Medium) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This MEM is modified as follows:
Without: L-glutamine, HEPES, Phenol Red
The complete formulation is available.
Using MEM
MEM was developed by Harry Eagle, and was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. MEM is available with Earle’s salts for use in a CO2 incubator, or with Hanks' salts for use without CO2. This product is made with Earle’s salts. MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Order Info
Shipping Condition: Room Temperature
Specifications
| Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
|
| Primary Rat Astrocytes | |
| 1 X | |
| MEM (Minimum Essential Medium) | |
| Sterile-filtered | |
| No Glutamine, No HEPES, No Phenol Red, No Sodium Pyruvate | |
| cGMP-compliant under the ISO 13485 standard | |
| 500 mL |
| HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts | |
| Animal Origin-free | |
| Liquid | |
| Standard Serum Supplementation | |
| Low Glucose | |
| Sustainable packaging | |
| Gibco™ | |
| Room Temperature |
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.