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Gibco™ McCoy's 5A (Modified) Medium
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McCoy's 5A (modified) Medium is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues.

Brand:  Gibco™ 16600082

389.35 NOK valid until 2025-03-29
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Product Code. 15410604

  • 584.00 NOK / 500mL

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Requires : Supplementation
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Description

Description

McCoy's 5A (modified) Medium is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues. This medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryos, and other tissues.

This McCoy's 5A is modified as follows:

With: High Glucose, L-glutamine, Bacto-peptone, Phenol Red

Without: Sodium Pyruvate, HEPES

The complete formulation is available.

Using McCoy's 5A (modified) Medium

Dr. Thomas McCoy originally formulated McCoy's 5A medium as a modification of Basal Medium 5A. Unlike other media, McCoy's 5A contains the reducing agent glutathione, bacto-peptone, and a high level of glucose. This product also includes Dr. Hsu's addition of Hanks' salts to enable use outside a CO2 incubator. McCoy's 5A (modified) Medium requires serum supplementation, commonly with 10% Fetal Bovine Serum (FBS). McCoy's 5A (modified) Medium uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Rat fibroblasts
Animal Origin
Liquid
Sterile-filtered
High Glucose, Glutamine, Phenol Red, Sodium Bicarbonate
cGMP-compliant under the ISO 13485 standard
500 mL
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Biopsy Tissues
1 X
McCoy's 5A Modified Medium
Sterile-filtered
No HEPES, No Sodium Pyruvate
Gibco™
Room Temperature
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