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Gibco™ DMEM, no glucose
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.
Brand: Gibco™ 11966025
Description
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This DMEM is modified as follows:
With: L-glutamine, Phenol Red
Without: Glucose, Sodium Pyruvate, HEPES
The complete formulation is available.
Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Order Info
Shipping Condition: Room Temperature
Specifications
| Storage conditions: 2°C to 8°C (protect from light) Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
|
| Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells | |
| 1 X | |
| DMEM (Dulbecco's Modified Eagle Medium) | |
| Sterile-filtered | |
| No Glucose, No HEPES, No Sodium Pyruvate | |
| Gibco™ | |
| Room Temperature |
| HeLa, 293, Cos-7, and PC-12 | |
| Animal Origin-free | |
| Liquid | |
| Standard Serum Supplementation | |
| Glutamine, Phenol Red | |
| cGMP-compliant under the ISO 13485 standard | |
| 500 mL |
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Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- o-Fluorotoluene
Signal Word
- Danger
Hazard Category
- Flammable liquid Category 2
- Specific target organ toxicity Category 3
Hazard Statement
- H225-Highly flammable liquid and vapour.
- H335-May cause respiratory irritation.
Precautionary Statement
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P240-Ground/bond container and receiving equipment.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
Supplemental information
- MIXTURE LIST-Contains : o-Fluorotoluene
Certificates
A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.
5 results found
| Lot Number | Certificate Type | Date | Catalog Number |
|---|---|---|---|
| 2740253 | Certificate of Analysis | 31/01/2025 | 11966025 |
| 2715486 | Certificate of Analysis | 29/01/2025 | 11966025 |
| 2750373 | Certificate of Analysis | 29/01/2025 | 11966025 |
| 2786646 | Certificate of Analysis | 28/01/2025 | 11966025 |
| 2821945 | Certificate of Analysis | 28/01/2025 | 11966025 |
Product Content Correction
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Product Title
Gibco™ DMEM, no glucose > Quantity: 500 mL
For Research Use Only. Not for use in diagnostic procedures.
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